Volume 9 Issue 6
Dec.  2023
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Xingrui Wang, Wenjuan Pu, Huan Zhu, Mingjun Zhang, Bin Zhou. Establishment of a Fah-LSL mouse model to study BEC-to-hepatocyte conversion. Biophysics Reports, 2023, 9(6): 309-324. doi: 10.52601/bpr.2023.230034
Citation: Xingrui Wang, Wenjuan Pu, Huan Zhu, Mingjun Zhang, Bin Zhou. Establishment of a Fah-LSL mouse model to study BEC-to-hepatocyte conversion. Biophysics Reports, 2023, 9(6): 309-324. doi: 10.52601/bpr.2023.230034

Establishment of a Fah-LSL mouse model to study BEC-to-hepatocyte conversion

doi: 10.52601/bpr.2023.230034
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  • Corresponding author: zhoubin@sibs.ac.cn (B. Zhou)
  • Received Date: 07 November 2023
  • Accepted Date: 22 January 2024
  • Available Online: 05 March 2024
  • Publish Date: 01 December 2023
  • The liver consists predominantly of hepatocytes and biliary epithelial cells (BECs), which serve distinct physiological functions. Although hepatocytes primarily replenish their own population during homeostasis and injury repair, recent findings have suggested that BECs can transdifferentiate into hepatocytes when hepatocyte-mediated liver regeneration is impaired. However, the cellular and molecular mechanisms governing this BEC-to-hepatocyte conversion remain poorly understood largely because of the inefficiency of existing methods for inducing lineage conversion. Therefore, this study introduces a novel mouse model engineered by the Zhou's lab, where hepatocyte senescence is induced by the deletion of the fumarylacetoacetate (Fah) gene. This model facilitates the efficient conversion of BECs to hepatocytes and allows for the simultaneous lineage tracing of BECs; consequently, a transitional liver progenitor cell population can be identified during lineage conversion. This study also outlines the technical procedures for utilizing this model to determine the underlying cellular and molecular mechanisms of BEC-to-hepatocyte conversion and provides new insights into liver regeneration and its underlying molecular mechanism.

  • The authors declare that they have no conflicts of interest.
    All institutional and national guidelines for the care and use of laboratory animals were followed.

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