Volume 9 Issue 6
Dec.  2023
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Jiangchao Xiang, Wenchao Xu, Jing Wu, Yaxin Luo, Bei Yang, Jia Chen. Nucleoside deaminases: the key players in base editing toolkit. Biophysics Reports, 2023, 9(6): 325-337. doi: 10.52601/bpr.2023.230029
Citation: Jiangchao Xiang, Wenchao Xu, Jing Wu, Yaxin Luo, Bei Yang, Jia Chen. Nucleoside deaminases: the key players in base editing toolkit. Biophysics Reports, 2023, 9(6): 325-337. doi: 10.52601/bpr.2023.230029

Nucleoside deaminases: the key players in base editing toolkit

doi: 10.52601/bpr.2023.230029
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  • The development of nucleoside deaminase-containing base editors realized targeted single base change with high efficiency and precision. Such nucleoside deaminases include adenosine and cytidine deaminases, which can catalyze adenosine-to-inosine (A-to-I) and cytidine-to-uridine (C-to-U) conversion respectively. These nucleoside deaminases are under the spotlight because of their vast application potential in gene editing. Recent advances in the engineering of current nucleoside deaminases and the discovery of new nucleoside deaminases greatly broaden the application scope and improve the editing specificity of base editors. In this review, we cover current knowledge about the deaminases used in base editors, including their key structural features, working mechanisms, optimization, and evolution.

  • Jiangchao Xiang, Wenchao Xu, Jing Wu, Yaxin Luo, Bei Yang and Jia Chen declare that they have no conflict of interest.
    This article does not contain any studies with human or animal subjects performed by any of the authors.
    Jiangchao Xiang, Wenchao Xu and Jing Wu contribute equally to this work.

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