Studying structure and functions of cell membranes by single molecule biophysical techniques

Qingrong Zhang; Siying Li; Yu Yang; Yuping Shan; Hongda Wang

doi:10.52601/bpr.2021.210018

Volume 7 Issue 5

Oct. 2021

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Qingrong Zhang, Siying Li, Yu Yang, Yuping Shan, Hongda Wang. Studying structure and functions of cell membranes by single molecule biophysical techniques[J]. Biophysics Reports, 2021, 7(5): 384-398. doi: 10.52601/bpr.2021.210018

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Qingrong Zhang, Siying Li, Yu Yang, Yuping Shan, Hongda Wang. Studying structure and functions of cell membranes by single molecule biophysical techniques[J]. Biophysics Reports, 2021, 7(5): 384-398. doi: 10.52601/bpr.2021.210018

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Studying structure and functions of cell membranes by single molecule biophysical techniques

doi: 10.52601/bpr.2021.210018

Qingrong Zhang^{1, 2},
Siying Li¹,
Yu Yang¹,
Yuping Shan^{1
,
,},
Hongda Wang^{2
,
,}

1.
School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Funds: This work was supported by National Key R&D Program of China (2017YFA0505300.), National Natural Science Foundation of China (21773017, 21727816 and 21721003).

More Information

Corresponding author: shanyp@ciac.ac.cn (Y. Shan); hdwang@ciac.ac.cn (H. Wang)
Received Date: 15 June 2021
Accepted Date: 11 August 2021
Publish Date: 31 October 2021

Abstract

Abstract

Cell membranes are complicated multicomponent structures, related to many basic cellular processes, such as substance transporting, energy conversion, signal transduction, mechanosensing, cell adhesion and so on. However, cell membranes have long been difficult to study at a single-molecule level due to their complex and dynamic properties. During the last decades, biophysical imaging techniques, such as atomic force microscopy and super-resolution fluorescent microscopy, have been developed to study biological structures with unprecedented resolution, enabling researchers to analyze the composition and distribution of membrane proteins and monitor their specific functions at single cell/molecule level. In this review, we highlight the structure and functions of cell membranes based on up-to-date biophysical techniques. Additionally, we describe the recent advances in force-based detecting technology, which allow insight into dynamic events and quantitativelymonitoring kinetic parameters for trans-membrane transporting in living cells.
- Cell membrane,
- Single molecule,
- Atomic force microscopy,
- Super-resolution fluorescence microscopy,
- Single molecule force spectroscopy

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References(155)

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