2021 Vol. 7, No. 5

Cover Story

Super-resolution imaging based on single-molecule localization has been developed for more than a decade. These techniques can break through diffraction limit of fluorescent microscopy and initially improve the resolu-tion by an order of magnitude to ~20 nm, by introducing photoactivatable/photoswitching probes and centroid fitting method. As the demand of bio-logical research, the localization precision of single-molecules was further improved by several state-of-the-art methods in the past several years. This review focuses on the latest developed techniques which have greatly im-proved the performance of single-molecule localization microscopy, from measurement principle to hardware design. These methods are essential for the study of nanostructures and biomacromolecule dynamics inside of cells.

Preparation of Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing of nucleic acids
Recent progress on single-molecule localization microscopy
Multiplexed single-molecule force spectroscopy for dissecting biophysical regulation of membrane receptors functions on live cells
Studying structure and functions of cell membranes by single molecule biophysical techniques
Protein folding mechanism revealed by single-molecule force spectroscopy experiments
Intracellular transport dynamics revealed by single-particle tracking