Volume 10 Issue 2
Apr.  2024
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Xiaochuan Fu, Shuyan Zhang, Pingsheng Liu. Co-immunoprecipitation for identifying protein–protein interaction on lipid droplets. Biophysics Reports, 2024, 10(2): 102-110. doi: 10.52601/bpr.2024.240007
Citation: Xiaochuan Fu, Shuyan Zhang, Pingsheng Liu. Co-immunoprecipitation for identifying protein–protein interaction on lipid droplets. Biophysics Reports, 2024, 10(2): 102-110. doi: 10.52601/bpr.2024.240007

Co-immunoprecipitation for identifying protein–protein interaction on lipid droplets

doi: 10.52601/bpr.2024.240007
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  • Corresponding author: syzhang@ibp.ac.cn (S. Zhang); pliu@ibp.ac.cn (P. Liu)
  • Received Date: 27 January 2024
  • Accepted Date: 23 February 2024
  • Available Online: 19 March 2024
  • Publish Date: 30 April 2024
  • The lipid droplet (LD) is a conserved organelle that exists in almost all organisms, ranging from bacteria to mammals. Dysfunctions in LDs are linked to a range of human metabolic syndromes. The formation of protein complexes on LDs is crucial for maintaining their function. Investigating how proteins interact on LDs is essential for understanding the role of LDs. We have developed an effective method to uncover protein–protein interactions and protein complexes specifically on LDs. In this method, we conduct co-immunoprecipitation (co-IP) experiments using LD proteins extracted directly from isolated LDs, rather than utilizing proteins from cell lysates. To elaborate, we begin by purifying LDs with high-quality and extracting LD-associated proteins. Subsequently, the co-IP experiment is performed on these LD-associated proteins directly, which would enhance the co-IP experiment specificity of LD-associated proteins. This method enables researchers to directly unveil protein complexes on LDs and gain deeper insights into the functional roles of proteins associated with LDs.

  • Xiaochuan Fu, Shuyan Zhang and Pingsheng Liu declare that they have no conflict of interest.
    All institutional and national guidelines for the care and use of laboratory animals were followed.

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