Volume 10 Issue 2
Apr.  2024
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Miao Ye, Yuting Chen, Zhaojie Liu, Yigang Wang, Cong Yi. Detection of ribophagy in yeast and mammals. Biophysics Reports, 2024, 10(2): 82-101. doi: 10.52601/bpr.2024.240002
Citation: Miao Ye, Yuting Chen, Zhaojie Liu, Yigang Wang, Cong Yi. Detection of ribophagy in yeast and mammals. Biophysics Reports, 2024, 10(2): 82-101. doi: 10.52601/bpr.2024.240002

Detection of ribophagy in yeast and mammals

doi: 10.52601/bpr.2024.240002
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  • Corresponding author: wangyigang43@163.com (Y. Wang); yiconglab@zju.edu.cn (C. Yi)
  • Received Date: 08 January 2024
  • Accepted Date: 01 February 2024
  • Available Online: 28 March 2024
  • Publish Date: 30 April 2024
  • Ribophagy, the cellular process wherein ribosomes are selectively self-digested through autophagy, plays a pivotal role in maintaining ribosome turnover. Understanding the molecular regulatory mechanisms governing ribophagy is pivotal to uncover its significance. Consequently, the establishment of methods for detecting ribophagy becomes important. In this protocol, we have optimized, enriched, and advanced existing ribophagy detection techniques, including immunoblotting, fluorescence microscopy, and transmission electron microscopy (TEM), to precisely monitor and quantify ribophagic events. Particularly noteworthy is the introduction of TEM technology for yeast ribophagy detection. In summary, the delineated methods are applicable for detecting ribophagy in both yeast and mammals, laying a solid foundation for further exploring the physiological importance of ribophagy and its potential implications in diverse cellular environments.

  • Yuting Chen, Miao Ye, Zhaojie Liu, Yigang Wang, and Cong Yi declare that they have no conflict of interest.
    This article does not contain any studies with human or animal subjects performed by the any of the authors.
    #Yuting Chen, Miao Ye and Zhaojie Liu contributed equally to this work.

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