Volume 10 Issue 1
Feb.  2024
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Qinggang Hao, Rui Dong, Weiyu Bai, Dong Chang, Xinyi Yao, Yingru Zhang, Huangying Xu, Huiyan Li, Xiang Kui, Feng Wang, Yan Wang, Chengqin Wang, Yujie Lei, Yan Chen, Junling Shen, Lei Sang, Yan Bai, Jianwei Sun. Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection. Biophysics Reports, 2024, 10(1): 15-21. doi: 10.52601/bpr.2023.230043
Citation: Qinggang Hao, Rui Dong, Weiyu Bai, Dong Chang, Xinyi Yao, Yingru Zhang, Huangying Xu, Huiyan Li, Xiang Kui, Feng Wang, Yan Wang, Chengqin Wang, Yujie Lei, Yan Chen, Junling Shen, Lei Sang, Yan Bai, Jianwei Sun. Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection. Biophysics Reports, 2024, 10(1): 15-21. doi: 10.52601/bpr.2023.230043

Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection

doi: 10.52601/bpr.2023.230043
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  • Tumor metastasis, responsible for approximately 90% of cancer-associated mortality, remains poorly understood. Here in this study, we employed a melanoma lung metastasis model to screen for metastasis-related genes. By sequential tail vein injection of mouse melanoma B16F10 cells and the subsequently derived cells from lung metastasis into BALB/c mice, we successfully obtained highly metastatic B16F15 cells after five rounds of in vivo screening. RNA-sequencing analysis of B16F15 and B16F10 cells revealed a number of differentially expressed genes, some of these genes have previously been associated with tumor metastasis while others are novel discoveries. The identification of these metastasis-related genes not only improves our understanding of the metastasis mechanisms, but also provides potential diagnostic biomarkers and therapeutic targets for metastatic melanoma.

  • Qinggang Hao, Rui Dong, Weiyu Bai, Dong Chang, Xinyi Yao, Yingru Zhang, Huangying Xu, Huiyan Li, Xiang Kui, Feng Wang, Yan Wang, Chengqin Wang, Yujie Lei, Yan Chen, Junling Shen, Lei Sang, Yan Bai and Jianwei Sun declare that they have no conflict of interest.
    All institutional and national guidelines for the care and use of laboratory animals were followed.
    Qinggang Hao, Rui Dong, Weiyu Bai and Dong Chang contributed equally to this work.

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