Volume 10 Issue 1
Feb.  2024
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Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu, Xiaofei Yang. An optimized method of culturing neurons based on polyacrylamide gel. Biophysics Reports, 2024, 10(1): 41-47. doi: 10.52601/bpr.2023.230033
Citation: Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu, Xiaofei Yang. An optimized method of culturing neurons based on polyacrylamide gel. Biophysics Reports, 2024, 10(1): 41-47. doi: 10.52601/bpr.2023.230033

An optimized method of culturing neurons based on polyacrylamide gel

doi: 10.52601/bpr.2023.230033
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  • Author Bio:

    Yongjing Qiao and Jihong Gong

  • Corresponding author: sunlittlefly@hotmail.com (X. Yang); jihonggong@foxmail.com (J. Gong)
  • Received Date: 02 November 2023
  • Accepted Date: 12 January 2024
  • Available Online: 05 March 2024
  • Publish Date: 29 February 2024
  • Substrate stiffness is a microenvironment with a certain stiffness constructed by the extracellular matrix and adjacent cells, which plays an important role in the growth and development of cells and tissue formation. Studies have indicated that the stiffness of the brain is about 0.1–1 kPa. The physiological and pathological processes of the nervous system are mediated by the substrate stiffness that the neurons suffer. However, how substrate stiffness regulates these processes remains to be studied. Culturing neurons on substrates with different stiffness in vitro is one of the best methods to study the role of stiffness in regulating neuronal development and activity. In this study, by changing the preparation time and the activation time of polyacrylamide gel, we provide an improved method that achieves a low toxic substrate environment for better primary neuron adhesion and development. Hope that this method is convenient for those studying the role of substrate stiffness in neurons.

  • Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu and Xiaofei Yang declare that they have no conflict of interest.
    All institutional and national guidelines for the care and use of laboratory animals were followed.
    Yongjing Qiao and Jihong Gong contributed equally to this work.

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