Volume 10 Issue 2
Apr.  2024
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Yiduo Liu, Junjie Hu, Bing Yan. Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation. Biophysics Reports, 2024, 10(2): 61-66. doi: 10.52601/bpr.2023.230031
Citation: Yiduo Liu, Junjie Hu, Bing Yan. Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation. Biophysics Reports, 2024, 10(2): 61-66. doi: 10.52601/bpr.2023.230031

Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation

doi: 10.52601/bpr.2023.230031
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  • Corresponding author: huj@ibp.ac.cn (J. Hu); bing_yan@ibp.ac.cn (B. Yan)
  • Received Date: 30 October 2023
  • Accepted Date: 15 December 2023
  • Available Online: 28 March 2024
  • Publish Date: 30 April 2024
  • The endoplasmic reticulum (ER) is an essential component of the endomembrane system in eukaryotes and plays a crucial role in protein and lipid synthesis, as well as the maintenance of calcium homeostasis. Morphologically, the ER is composed primarily of sheets and tubules. The tubular ER is composed of a network of tubular membrane structures, each with diameters ranging from 30 to 50 nanometers. In recent years, there has been in-depth research on the molecular mechanisms of membrane shaping and membrane fusion of the tubular ER. However, there is still limited understanding of the specific physiological functions of the tubular ER. Here, we report a protocol that combines differential centrifugation and immunoprecipitation to specifically enrich microsomes originating from the tubular ER in yeast. The ER tubule-derived microsomes can be further used for proteomic and lipidomic studies or other biochemical analyses.

  • Yiduo Liu, Junjie Hu and Bing Yan declare that they have no conflict of interest.
    This article does not contain any studies with human or animal subjects performed by any of the authors.

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