Volume 10 Issue 2
Apr.  2024
Turn off MathJax
Article Contents
Yuwei Huang, Li Yu. Seeing is believing: observation of migrasomes. Biophysics Reports, 2024, 10(2): 67-81. doi: 10.52601/bpr.2023.230024
Citation: Yuwei Huang, Li Yu. Seeing is believing: observation of migrasomes. Biophysics Reports, 2024, 10(2): 67-81. doi: 10.52601/bpr.2023.230024

Seeing is believing: observation of migrasomes

doi: 10.52601/bpr.2023.230024
More Information
  • Corresponding author: liyulab@mail.tsinghua.edu.cn (L. Yu)
  • Received Date: 22 October 2023
  • Accepted Date: 29 December 2023
  • Available Online: 05 March 2024
  • Publish Date: 30 April 2024
  • Migrasomes are a novel type of cell organelle that form on the retraction fibers at the rear of migrating cells. In recent years, numerous studies have unveiled the mechanisms of migrasome formation and have highlighted significant roles of migrasomes in both physiological and pathological processes. Building upon the strategies outlined in published works and our own research experiences, we have compiled a comprehensive set of protocols for observing migrasomes. These step-by-step instructions encompass various aspects such as cell culture, labeling, imaging, in vitro reconstitution, and statistical analysis. We believe that these protocols serve as a valuable resource for researchers exploring migrasome biology.

  • Yuwei Huang and Li Yu declare that they have no conflict of interest.
    This article does not contain any studies with human or animal subjects performed by any of the authors.

  • loading
  • Dharan R, Goren S, Cheppali SK, Shendrik P, Brand G, Vaknin A, Yu L, Kozlov MM, Sorkin R (2022) Transmembrane proteins tetraspanin 4 and CD9 sense membrane curvature. Proc Natl Acad Sci USA 119(43): e2208993119. https://doi.org/10.1073/pnas.2208993119
    Dharan R, Huang Y, Cheppali SK, Goren S, Shendrik P, Wang W, Qiao J, Kozlov MM, Yu L, Sorkin R (2023) Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes. Nat Commun 14(1): 1037. https://doi.org/10.1038/s41467-023-36596-9
    Ding T, Ji J, Zhang W, Liu Y, Liu B, Han Y, Chen C, Yu L (2023) The phosphatidylinositol (4, 5)-bisphosphate-Rab35 axis regulates migrasome formation. Cell Res 33(8): 617−627 doi: 10.1038/s41422-023-00811-5
    Fan C, Shi X, Zhao K, Wang L, Shi K, Liu YJ, Li H, Ji B, Jiu Y (2022) Cell migration orchestrates migrasome formation by shaping retraction fibers. J Cell Biol 221(4): e202109168. https://doi.org/10.1083/jcb.202109168
    Hu M, Li T, Ma X, Liu S, Li C, Huang Z, Lin Y, Wu R, Wang S, Lu D, Lu T, Men X, Shen S, Huang H, Liu Y, Song K, Jian B, Jiang Y, Qiu W, Liu Q, Lu Z, Cai W (2023) Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model. Nat Commun 14(1): 3945. https://doi.org/10.1038/s41467-023-39693-x
    Huang Y, Zucker B, Zhang S, Elias S, Zhu Y, Chen H, Ding T, Li Y, Sun Y, Lou J, Kozlov MM, Yu L (2019) Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains. Nat Cell Biol 21(8): 991−1002 doi: 10.1038/s41556-019-0367-5
    Jiang D, Jiang Z, Lu D, Wang X, Liang H, Zhang J, Meng Y, Li Y, Wu D, Huang Y, Chen Y, Deng H, Wu Q, Xiong J, Meng A, Yu L (2019) Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation. Nat Cell Biol 21(8): 966−977 doi: 10.1038/s41556-019-0358-6
    Jiao H, Jiang D, Hu X, Du W, Ji L, Yang Y, Li X, Sho T, Wang X, Li Y, Wu YT, Wei YH, Hu X, Yu L (2021) Mitocytosis, a migrasome-mediated mitochondrial quality-control process. Cell 184(11): 2896−2910 e2813
    Liang H, Ma X, Zhang Y, Liu Y, Liu N, Zhang W, Chen J, Liu B, Du W, Liu X, Yu L (2023) The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat Cell Biol 25(8): 1173−1184 doi: 10.1038/s41556-023-01188-8
    Liu Y, Li S, Rong W, Zeng C, Zhu X, Chen Q, Li L, Liu ZH, Zen K (2020) Podocyte-released migrasomes in urine serve as an indicator for early podocyte injury. Kidney Dis (Basel) 6(6): 422−433 doi: 10.1159/000511504
    Ma L, Li Y, Peng J, Wu D, Zhao X, Cui Y, Chen L, Yan X, Du Y, Yu L (2015) Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res 25(1): 24−38 doi: 10.1038/cr.2014.135
    Schmidt-Pogoda A, Strecker JK, Liebmann M, Massoth C, Beuker C, Hansen U, Konig S, Albrecht S, Bock S, Breuer J, Sommer C, Schwab N, Wiendl H, Klotz L, Minnerup J (2018) Dietary salt promotes ischemic brain injury and is associated with parenchymal migrasome formation. PLoS One 13(12): e0209871. https://doi.org/10.1371/journal.pone.0209871
    Wang M, Xiong C, Mercurio AM (2022) PD-LI promotes rear retraction during persistent cell migration by altering integrin beta4 dynamics. J Cell Biol 221(5): e202108083. https://doi.org/10.1083/jcb.202108083
    Wu D, Xu Y, Ding T, Zu Y, Yang C, Yu L (2017) Pairing of integrins with ECM proteins determines migrasome formation. Cell Res 27(11): 1397−1400 doi: 10.1038/cr.2017.108
    Wu J, Lu Z, Jiang D, Guo Y, Qiao H, Zhang Y, Zhu T, Cai Y, Zhang X, Zhanghao K, Xie H, Yan T, Zhang G, Li X, Jiang Z, Lin X, Fang L, Zhou B, Xi P, Fan J, Yu L, Dai Q (2021) Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale. Cell 184(12): 3318−3332 e3317
    Wu L, Yang S, Li H, Zhang Y, Feng L, Zhang C, Wei J, Gu X, Xu G, Wang Z, Wang F (2022) TSPAN4-positive migrasome derived from retinal pigmented epithelium cells contributes to the development of proliferative vitreoretinopathy. J Nanobiotechnology 20(1): 519. https://doi.org/10.1186/s12951-022-01732-y
    Zhang C, Li T, Yin S, Gao M, He H, Li Y, Jiang D, Shi M, Wang J, Yu L (2022) Monocytes deposit migrasomes to promote embryonic angiogenesis. Nat Cell Biol 24(12): 1726−1738 doi: 10.1038/s41556-022-01026-3
    Zhao X, Lei Y, Zheng J, Peng J, Li Y, Yu L, Chen Y (2019) Identification of markers for migrasome detection. Cell Discov 5: 27. https://doi.org/10.1038/s41421-019-0093-y
    Zhu M, Zou Q, Huang R, Li Y, Xing X, Fang J, Ma L, Li L, Yang X, Yu L (2021) Lateral transfer of mRNA and protein by migrasomes modifies the recipient cells. Cell Res 31(2): 237−240 doi: 10.1038/s41422-020-00415-3
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(9)  / Tables(3)

    Article Metrics

    Article views (290) PDF downloads(34) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return