Cell membrane sample preparation method of combined AFM and dSTORM analysis

Mingjun Cai; Huili Wang; Guanfang Zhao; Hongru Li; Jing Gao; Hongda Wang

doi:10.52601/bpr.2022.220004

Volume 8 Issue 4

Aug. 2022

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Article Navigation > Biophysics Reports > 2022 > 8(4): 183-192

Mingjun Cai, Huili Wang, Guanfang Zhao, Hongru Li, Jing Gao, Hongda Wang. Cell membrane sample preparation method of combined AFM and dSTORM analysis[J]. Biophysics Reports, 2022, 8(4): 183-192. doi: 10.52601/bpr.2022.220004

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Mingjun Cai, Huili Wang, Guanfang Zhao, Hongru Li, Jing Gao, Hongda Wang. Cell membrane sample preparation method of combined AFM and dSTORM analysis[J]. Biophysics Reports, 2022, 8(4): 183-192. doi: 10.52601/bpr.2022.220004

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Cell membrane sample preparation method of combined AFM and dSTORM analysis

doi: 10.52601/bpr.2022.220004

Mingjun Cai^{1, 2
,
,},
Huili Wang²,
Guanfang Zhao^{1, 2},
Hongru Li^{1, 2},
Jing Gao²,
Hongda Wang^{1, 2, 3
,
,}

1.
University of Science and Technology of China, Hefei 230027, China
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
3.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, Shandong, China

More Information

Corresponding author: caimingjun@ciac.ac.cn (M. Cai); hdwang@ciac.ac.cn (H. Wang)
Received Date: 31 January 2022
Accepted Date: 06 April 2022

Available Online: 25 July 2022

Publish Date: 31 August 2022

Abstract

Abstract

A major role of cell membranes is to provide an ideal environment for the constituent proteins to perform their biological functions. A deep understanding of the membrane proteins assembly process under physiological conditions is quite important to elucidate both the structure and the function of the cell membranes. Along these lines, in this work, a complete workflow of the cell membrane sample preparation and the correlated AFM and dSTORM imaging analysis methods are presented. A specially designed, angle-controlled sample preparation device was used to prepare the cell membrane samples. The correlated distributions of the specific membrane proteins with the topography of the cytoplasmic side of the cell membranes can be obtained by performing correlative AFM and dSTORM measurements. These methods are ideal for systematically studying the structure of the cell membranes. The proposed method of the sample characterization was not only limited to the measurement of the cell membrane but also can be applied for both biological tissue section analysis and detection.

Mingjun Cai, Huili Wang, Guanfang Zhao, Hongru Li, Jing Gao and Hongda Wang declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by the any of the authors.

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References(25)

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