Chick chorioallantoic membrane model to investigate role of migrasome in angiogenensis

Cuifang Zhang; Helen He; Shuyao Yin; Mingyi Gao; Li Yu

doi:10.52601/bpr.2023.230021

Volume 9 Issue 5

Oct. 2023

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Cuifang Zhang, Helen He, Shuyao Yin, Mingyi Gao, Li Yu. Chick chorioallantoic membrane model to investigate role of migrasome in angiogenensis. Biophysics Reports, 2023, 9(5): 241-254. doi: 10.52601/bpr.2023.230021

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Cuifang Zhang, Helen He, Shuyao Yin, Mingyi Gao, Li Yu. Chick chorioallantoic membrane model to investigate role of migrasome in angiogenensis. Biophysics Reports, 2023, 9(5): 241-254. doi: 10.52601/bpr.2023.230021

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Chick chorioallantoic membrane model to investigate role of migrasome in angiogenensis

doi: 10.52601/bpr.2023.230021

Cuifang Zhang^{1, 2},
Helen He¹,
Shuyao Yin¹,
Mingyi Gao¹,
Li Yu^{1
,
,}

1.
The State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, Beijing 100084, China
2.
Xiamen Cardiovascular Hospital, Xiamen University, Xiamen 361005, Fujian, China

More Information

Corresponding author: liyulab@mail.tsinghua.edu.cn (L. Yu)
Received Date: 08 November 2023
Accepted Date: 28 November 2023

Available Online: 26 February 2024

Publish Date: 01 October 2023

Abstract

Abstract

The development of the vascular system is essential for embryonic development, including processes such as angiogenesis. Angiogenesis plays a critical role in many normal physiological and pathological processes. It is driven by a set of angiogenic proteins, including angiogenic growth factors, chemokines, and extracellular matrix proteins. Among various animal model systems, the chorioallantoic membrane (CAM), a specialized and highly vascularized tissue of the avian embryo, has proven to be a valuable tool for analyzing the angiogenic potential of candidate cells or factors. In this protocol, we provide detailed procedures for establishing the CAM model to evaluate the function and mechanism of migrasomes in embryonic angiogenesis. This includes the CAM nylon mesh assay and CAM ex vivo sprouting assay to assess CAM angiogenesis, as well as the observation, purification, and delivery of migrasomes. Additionally, we describe the generation of T4-KO-mCherry-KI embryos using the CRISPR system within the CAM tissue to investigate the role of migrasomes in angiogenesis.
- Angiogenesis,
- Chorioallantoic membrane (CAM),
- Chick CAM model

Li Yu is the scientific founder of Migrasome therapeutics Ltd, and Cuifang Zhang, Helen He, Shuyao Yin and Mingyi Gao declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.

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References(11)

References

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