<i>Xenopus</i> cell-free extracts and their applications in cell biology study

Junjun Liu; Chuanmao Zhang

doi:10.52601/bpr.2023.230016

Volume 9 Issue 4

Aug. 2023

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Article Navigation > Biophysics Reports > 2023 > 9(4): 195-205

Junjun Liu, Chuanmao Zhang. Xenopus cell-free extracts and their applications in cell biology study. Biophysics Reports, 2023, 9(4): 195-205. doi: 10.52601/bpr.2023.230016

Citation:

Junjun Liu, Chuanmao Zhang. Xenopus cell-free extracts and their applications in cell biology study. Biophysics Reports, 2023, 9(4): 195-205. doi: 10.52601/bpr.2023.230016

Junjun Liu, Chuanmao Zhang. Xenopus cell-free extracts and their applications in cell biology study. Biophysics Reports, 2023, 9(4): 195-205. doi: 10.52601/bpr.2023.230016

Citation:

Junjun Liu, Chuanmao Zhang. Xenopus cell-free extracts and their applications in cell biology study. Biophysics Reports, 2023, 9(4): 195-205. doi: 10.52601/bpr.2023.230016

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Xenopus cell-free extracts and their applications in cell biology study

doi: 10.52601/bpr.2023.230016

Junjun Liu¹,
Chuanmao Zhang^{2, 3
,
,}

1.
Department of Biological Sciences, California State Polytechnic University, Pomona, CA 91768, USA
2.
The Academy for Cell and Life Health, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
3.
The Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China

More Information

Corresponding author: zhangcm@kust.edu.cn or zhangcm@pku.edu.cn (C. Zhang)
Received Date: 27 September 2023
Accepted Date: 05 December 2023

Available Online: 26 February 2024

Publish Date: 31 August 2023

Abstract

Abstract

Xenopus has proven to be a remarkably versatile model organism in the realm of biological research for numerous years, owing to its straightforward maintenance in laboratory settings and its abundant provision of ample-sized oocytes, eggs, and embryos. The cell cycle of these oocytes, eggs, and early embryos exhibits synchrony, and extracts derived from these cells serve various research purposes. Many fundamental concepts in biochemistry, cell biology, and development have been elucidated through the use of cell-free extracts derived from Xenopus cells. Over the past few decades, a wide array of cell-free extracts has been prepared from oocytes, eggs, and early embryos of different Xenopus species at varying cell cycle stages. Each of these extracts possesses distinct characteristics. This review provides a concise overview of the Xenopus species employed in laboratory research, the diverse types of cell-free extracts available, and their respective properties. Furthermore, this review delves into the extensive investigation of spindle assembly in Xenopus egg extracts, underscoring the versatility and potency of these cell-free systems in the realm of cell biology.
- Xenopus,
- Cell-free extract,
- Oocyte,
- Egg,
- Embryo,
- Meiosis,
- Mitosis,
- Spindle

Junjun Liu and Chuanmao Zhang declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.

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References(69)

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