Isolation and proteomic study of fish liver lipid droplets

Yuwei Sun; Jian Heng; Feng Liu; Shuyan Zhang; Pingsheng Liu

doi:10.52601/bpr.2023.230004

Volume 9 Issue 3

Jun. 2023

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Article Navigation > Biophysics Reports > 2023 > 9(3): 120-133

Yuwei Sun, Jian Heng, Feng Liu, Shuyan Zhang, Pingsheng Liu. Isolation and proteomic study of fish liver lipid droplets. Biophysics Reports, 2023, 9(3): 120-133. doi: 10.52601/bpr.2023.230004

Citation:

Yuwei Sun, Jian Heng, Feng Liu, Shuyan Zhang, Pingsheng Liu. Isolation and proteomic study of fish liver lipid droplets. Biophysics Reports, 2023, 9(3): 120-133. doi: 10.52601/bpr.2023.230004

Yuwei Sun, Jian Heng, Feng Liu, Shuyan Zhang, Pingsheng Liu. Isolation and proteomic study of fish liver lipid droplets. Biophysics Reports, 2023, 9(3): 120-133. doi: 10.52601/bpr.2023.230004

Citation:

Yuwei Sun, Jian Heng, Feng Liu, Shuyan Zhang, Pingsheng Liu. Isolation and proteomic study of fish liver lipid droplets. Biophysics Reports, 2023, 9(3): 120-133. doi: 10.52601/bpr.2023.230004

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Isolation and proteomic study of fish liver lipid droplets

doi: 10.52601/bpr.2023.230004

Yuwei Sun^{1, 4},
Jian Heng^{2, 3},
Feng Liu^{2, 3, 4},
Shuyan Zhang^{1
,
,},
Pingsheng Liu^{1, 4
,
,}

1.
National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2.
State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
3.
Institute of Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China
4.
University of Chinese Academy of Sciences, Beijing 100049, China

More Information

Corresponding author: syzhang@ibp.ac.cn ( S. Zhang); pliu@ibp.ac.cn (P. Liu)
Received Date: 26 February 2023
Accepted Date: 02 June 2023

Available Online: 03 November 2023

Publish Date: 01 June 2023

Abstract

Abstract

Lipid droplets (LDs) are a neutral lipid storage organelle that is conserved in almost all species. Excessive storage of neutral lipids in LDs is directly associated with many metabolic syndromes. Zebrafish is a better model animal for the study of LD biology due to its transparent embryonic stage compared to other organisms. However, the study of LDs in fish has been difficult due to the lack of specific LD marker proteins and the limitation of purification technology. In this paper, the purification and proteomic analysis of liver LDs of fish including zebrafish and Carassius auratus were performed for the first time. 259 and 267 proteins were identified respectively. Besides most of the identified proteins were reported in previous LD proteomes of mammals, indicating the similarity between mammal and fish LDs. We also identified many unique proteins of liver LDs in fish that are involved in the regulation of LD dynamics. Through morphological and biochemical analysis, we found that the marker protein Plin2 of zebrafish LD was located on LDs in Huh7 cells. These results will facilitate further study of LDs in fish and liver metabolic diseases using fish as a model animal.
- Lipid droplet,
- Fish,
- Liver,
- Proteomics,
- Plin2

Yuwei Sun, Jian Heng, Feng Liu, Shuyan Zhang and Pingsheng Liu declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.

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References(78)

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