Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

Xiaodan Lu; Yifan Lin; Xiuying Lin; Qiang Zhang; Zihang Wang; Xuguang Mi; Ruobing Wang; Xiaofang Zhang; Xu Luan; Yan Liu; Bing Li; Yan Tan; Yanqiu Fang

doi:10.52601/bpr.2021.200046

Volume 7 Issue 2

Feb. 2021

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Article Navigation > Biophysics Reports > 2021 > 7(2): 142-151

Xiaodan Lu, Yifan Lin, Xiuying Lin, Qiang Zhang, Zihang Wang, Xuguang Mi, Ruobing Wang, Xiaofang Zhang, Xu Luan, Yan Liu, Bing Li, Yan Tan, Yanqiu Fang. Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells[J]. Biophysics Reports, 2021, 7(2): 142-151. doi: 10.52601/bpr.2021.200046

Citation:

Xiaodan Lu, Yifan Lin, Xiuying Lin, Qiang Zhang, Zihang Wang, Xuguang Mi, Ruobing Wang, Xiaofang Zhang, Xu Luan, Yan Liu, Bing Li, Yan Tan, Yanqiu Fang. Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells[J]. Biophysics Reports, 2021, 7(2): 142-151. doi: 10.52601/bpr.2021.200046

Citation:

Xiaodan Lu, Yifan Lin, Xiuying Lin, Qiang Zhang, Zihang Wang, Xuguang Mi, Ruobing Wang, Xiaofang Zhang, Xu Luan, Yan Liu, Bing Li, Yan Tan, Yanqiu Fang. Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells[J]. Biophysics Reports, 2021, 7(2): 142-151. doi: 10.52601/bpr.2021.200046

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Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

doi: 10.52601/bpr.2021.200046

Xiaodan Lu^{1, 2
,
,},
Yifan Lin^{1, 2},
Xiuying Lin¹,
Qiang Zhang³,
Zihang Wang⁴,
Xuguang Mi¹,
Ruobing Wang⁴,
Xiaofang Zhang^{1, 2},
Xu Luan^{1, 2},
Yan Liu^{1, 2},
Bing Li³,
Yan Tan¹,
Yanqiu Fang^{1, 2
,
,}

1.
Diagnostic Medical Center, Jilin Province People's Hospital, Changchun 130021, China
2.
School of Medicine, Changchun University of Chinese Medicine, Changchun 130021, China
3.
Department of Clinical Laboratory, the Second Hospital of Jilin University, Changchun 130041, China
4.
General Surgery, the First Hospital of Jilin University, Changchun 130021, China

Funds: This study was funded by Jilin Province Science and Technology Fund (20180201028YY, 20190201239JC, 20200403081SF and 20190304042YY), the National Natural Science Foundation of China (81970628, 32011540004), Jilin Talents Developing Fund (2019026), and Health Commission of Jilin Province (2019J069, 2018J048, 2017J019 and JJKH20190056KJ). Y. Fang, Y. Tan and X. Lu designed and conceived research; Y. Liu, X. Lin, R. Wang and X. Zhang performed experiments; B. Li and X. Mi analyzed the data; X. Luan, Q. Zhang, Z. Wang and Y. Liu wrote the manuscript.

More Information

Corresponding author: luxiaodan@ccsfu.edu.cn (X. Lu); yq.fang@163.com (Y. Fang)
Received Date: 11 September 2020
Accepted Date: 05 March 2021

Available Online: 17 May 2021

Publish Date: 28 February 2021

Abstract

Abstract

Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent and functional in many biological processes, by which releasing secretary factors to promote the self-repairing of damaged tissue or developing into functional cell at local organ. However, there is a high risk that oxidative stress would reduce the pluripotency and factor-secretion during the preparation and transplantation. Therefore, reducing oxidative stress is expected to improve the efficacy of HUC-MSCs therapy. Zinc (Zn) is an essential trace element which involves in the resistance of oxidative stress. To investigate Zn-regulated signaling pathways, we have profiled the gene expression at transcriptome level in primary HUC-MSCs treated with zinc sulfate, followed with GO and KEGG gene enrichment analysis. Zn treatment improved signal pathways for mineral absorption, cell growth, and cell death. Zn deficiency was mimicked by TPEN administration, which suppressed cell proliferation and reduced the expression of HUC-MSCs surface stem cell markers CD73, CD90 and CD105 by flow cytometry. Nuclear factor erythrocyte 2 related factor 2 (Nrf2) plays an important role in antioxidant biological processes. In vitro treatment of Zn significantly increased Nrf2 and Sirt3 expression at gene level and protein level respectively. Zn supplementation inhibited TPEN-induced failure of cell survival and reversed the reduction of Nrf2 and Sirt3 expression, which further reduced the production of ROS. Zn successfully presented its anti-oxidation effect by activating Nrf2/Sirt3 signaling pathway in HUC-MSCs. Zn supplementation may improve the efficacy of HUC-MSCs therapy with reduced oxidative stress.
- Zinc,
- Oxidative stress,
- Nuclear factor erythroid-2-related factor 2,
- Sirtuin 3,
- Human umbilical cord mesenchymal stem cells

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References(34)

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