Analysis of chromatin bersin Hela cells with electron tomography

Xiaomin Li; Hongli Feng; Jianguo Zhang; Lei Sun; Ping Zhu

doi:10.1007/x41048-015-0009-9

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Xiaomin Li, Hongli Feng, Jianguo Zhang, Lei Sun, Ping Zhu. Analysis of chromatin bersin Hela cells with electron tomography. Biophysics Reports, 2015, 1(1): 51-60. doi: 10.1007/x41048-015-0009-9

Citation:

Xiaomin Li, Hongli Feng, Jianguo Zhang, Lei Sun, Ping Zhu. Analysis of chromatin bersin Hela cells with electron tomography. Biophysics Reports, 2015, 1(1): 51-60. doi: 10.1007/x41048-015-0009-9

Xiaomin Li, Hongli Feng, Jianguo Zhang, Lei Sun, Ping Zhu. Analysis of chromatin bersin Hela cells with electron tomography. Biophysics Reports, 2015, 1(1): 51-60. doi: 10.1007/x41048-015-0009-9

Citation:

Xiaomin Li, Hongli Feng, Jianguo Zhang, Lei Sun, Ping Zhu. Analysis of chromatin bersin Hela cells with electron tomography. Biophysics Reports, 2015, 1(1): 51-60. doi: 10.1007/x41048-015-0009-9

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Analysis of chromatin bersin Hela cells with electron tomography

doi: 10.1007/x41048-015-0009-9

1 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 China
2 Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
3 University of Chinese Academy of Sciences, Beijing 100049, China

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Corresponding author: Ping Zhu
Received Date: 09 March 2015
Rev Recd Date: 12 April 2015
Publish Date: 31 August 2015

Abstract

Abstract

The presence and folding pattern of chromatin in eukaryotic cells remain elusive and controversial. In this study, we prepared ultra-thin sections of Hela cells with three different fixation and sectioning methods, i.e., chemical fixation, high pressure freezing with freeze substitution, and cryo-ultramicro- tomy with SEM-FIB (focused ion beam), and analyzed in vivo architecture of chromatin fibers in Hela nuclei with electron tomography technology. The results suggest that the chromatin fibers in eukaryotic Hela cells are likely organized in an architecture with a diameter of about 30 nm.
- Chromatin fiber, Hela cell,
- Electron tomography,
- Chemical fixation,
- High-pressure freezing,
- Cryoultramicrotomy,
- DualBeam-FIB

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References(38)

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