Two chemical-controlled switchable Cas9s for tunable gene editing

Meng Liang; Yang Cui; Jie Lan; Guangtao Song; Jizhong Lou

doi:10.1007/s41048-019-0093-3

Volume 5 Issue 3

Turn off MathJax

Article Contents

Article Navigation > Biophysics Reports > 2019 > 5(3): 161-165

Meng Liang, Yang Cui, Jie Lan, Guangtao Song, Jizhong Lou. Two chemical-controlled switchable Cas9s for tunable gene editing. Biophysics Reports, 2019, 5(3): 161-165. doi: 10.1007/s41048-019-0093-3

Citation:

Meng Liang, Yang Cui, Jie Lan, Guangtao Song, Jizhong Lou. Two chemical-controlled switchable Cas9s for tunable gene editing. Biophysics Reports, 2019, 5(3): 161-165. doi: 10.1007/s41048-019-0093-3

Meng Liang, Yang Cui, Jie Lan, Guangtao Song, Jizhong Lou. Two chemical-controlled switchable Cas9s for tunable gene editing. Biophysics Reports, 2019, 5(3): 161-165. doi: 10.1007/s41048-019-0093-3

Citation:

Meng Liang, Yang Cui, Jie Lan, Guangtao Song, Jizhong Lou. Two chemical-controlled switchable Cas9s for tunable gene editing. Biophysics Reports, 2019, 5(3): 161-165. doi: 10.1007/s41048-019-0093-3

PDF (1082 KB)

Two chemical-controlled switchable Cas9s for tunable gene editing

doi: 10.1007/s41048-019-0093-3

Meng Liang^1,2,
Yang Cui^1,2,
Jie Lan^1,2,
Guangtao Song^{1,2
,
,},
Jizhong Lou^{1,2
,}

1 Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2 University of Chinese Academy of Sciences, Beijing 100049, China

Funds: This work was supported by the National Natural Science Foundation of China (31771015, 11672317).

More Information

Corresponding author: Guangtao Song, Jizhong Lou
Received Date: 21 January 2019
Publish Date: 30 June 2019

Abstract

FullText(HTML)

References(18)

References

Banaszynski LA, Chen L-C, Maynard-Smith LA, Ooi AGL, Wandless TJ (2006) A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell 126:995-1004

Beal MF (1995) Aging, energy, and oxidative stress in neurodegenerative diseases. Ann Neurol 38:357-366

Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, Terskikh AV, Lukyanov S (2006) Genetically encoded fluorescent indicator for intracellular hydrogen peroxide. Nat Methods 3:281-286

Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339:819-823

ElrodErickson M, Rould MA, Nekludova L, Pabo CO (1996) Zif268 protein-DNA complex refined at 1.6 angstrom:a model system for understanding zinc finger-DNA interactions. Structure 4:1171-1180

Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD (2013) High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol 31:822-826

Fu YF, Sander JD, Reyon D, Cascio VM, Joung JK (2014) Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol 32:279-284

Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS (2016) Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods 13:1043-1049

Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F (2013) DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol 31:827-832

Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA (2016) Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage. Science 351:867-871

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816-821

Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng ZL, Joung JK (2016) High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature 529:490-495

Mohanraju P, Makarova KS, Zetsche B, Zhang F, Koonin EV, van der Oost J (2016) Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems. Science 353:aad5147

Nihongaki Y, Otabe T, Sato M (2018) Emerging approaches for spatiotemporal control of targeted genome with inducible CRISPR-Cas9. Anal Chem 90:429-439

Richter F, Fonfara I, Gelfert R, Nack J, Charpentier E, Moglich A (2017) Switchable Cas9. Curr Opin Biotechnol 48:119-126

Simonian NA, Coyle JT (1996) Oxidative stress in neurodegenerative diseases. Annu Rev Pharmacol Toxicol 36:83-106

Tycko J, Myer VE, Hsu PD (2016) Methods for optimizing CRISPRCas9 genome editing specificity. Mol Cell 63:355-370

Zeng Y, Cui Y, Zhang Y, Zhang Y, Liang M, Chen H, Lan J, Song G, Lou J (2018) The initiation, propagation and dynamics of CRISPRSpyCas9 R-loop complex. Nucleic Acids Res 46:350-361

Supplements(0)

Relative Articles

Cited By

Proportional views