Global mapping of live cell mechanical features using PeakForce QNM AFM

Jinrong Hu; Shenbao Chen; Dandan Huang; Yan Zhang; Shouqin Lü Mian Long

doi:10.1007/s41048-019-00103-9

Volume 6 Issue 1

Turn off MathJax

Article Contents

Article Navigation > Biophysics Reports > 2020 > 6(1): 9-18

Jinrong Hu, Shenbao Chen, Dandan Huang, Yan Zhang, Shouqin Lü Mian Long. Global mapping of live cell mechanical features using PeakForce QNM AFM. Biophysics Reports, 2020, 6(1): 9-18. doi: 10.1007/s41048-019-00103-9

Citation:

Jinrong Hu, Shenbao Chen, Dandan Huang, Yan Zhang, Shouqin Lü Mian Long. Global mapping of live cell mechanical features using PeakForce QNM AFM. Biophysics Reports, 2020, 6(1): 9-18. doi: 10.1007/s41048-019-00103-9

Citation:

PDF (2838 KB)

Global mapping of live cell mechanical features using PeakForce QNM AFM

doi: 10.1007/s41048-019-00103-9

1 Center of Biomechanics and Bioengineering, Key Laboratory of Microgravity(National Microgravity Laboratory), Beijing Key Laboratory of Engineered Construction and Mechanobiology, and CAS Center for Excellence in Complex System Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China
2 School of Engineering Science, University of Chinese Academy of Sciences, Beijing 100049, China

Funds: Shouqin Lü, Mian Long

Received Date: 12 June 2019
Rev Recd Date: 25 September 2019
Publish Date: 29 February 2020

Abstract

Abstract

Atomic force microscopy (AFM) has been widely used to acquire surface topography upon different scanning modes and to quantify mechanical properties of a cell using single-point ramp force mode. However, these traditional measurements need massive force curves originating from multiple points of a cell to exclude the potential errors resulted from limited and factitious selections of testing points, making the measurements time-consuming and highly localized. PeakForce Quantitative NanoMechanics (PF QNM) is a high-speed (faster by 3-4 order of magnitude) and global surface mechanical property mapping method with high spatial resolution, overcoming the drawbacks of traditional ramp mode especially used for a live cell with high heterogeneity. In this protocol, we elaborated how to run PF QNM measurements for live cells and relevant modification may be needed when extending this method to other cell-like soft materials.
- Global mechanical mapping,
- Live cell,
- Atomic force microscopy,
- PeakForce QNM

FullText(HTML)

References(35)

References

Åström KJ, Hägglund T (1995) PID controllers:theory, design, and tuning, vol 2. Instrument Society of America, Research Triangle Park

Barbee KA, Davies PF, Lal R (1994) Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy. Circ Res 74:163-171

Calzado-Martín A, Encinar M, Tamayo J, Calleja M, San Paulo A (2016) Effect of actin organization on the stiffness of living breast cancer cells revealed by peak-force modulation atomic force microscopy. ACS Nano 10:3365-3374

Chaudhuri O, Parekh SH, Fletcher DA (2007) Reversible stress softening of actin networks. Nature 445:295-298

Curry N, Ghézali G, Kaminski Schierle GS, Rouach N, Kaminski CF (2017) Correlative STED and atomic force microscopy on live astrocytes reveals plasticity of cytoskeletal structure and membrane physical properties during polarized migration. Front Cell Neurosci 11:104

Derjaguin BV, Muller VM, Toporov YP (1975) Effect of contact deformations on the adhesion of particles. J Colloid Interf Sci 53:314-326

Efremov YM, Cartagena-Rivera AX, Athamneh AI, Suter DM, Raman A (2018) Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy. Nat Protoc 13:2200

Galluzzi M, Biswas CS, Wu Y, Wang Q, Du B, Stadler FJ (2016) Space-resolved quantitative mechanical measurements of soft and supersoft materials by atomic force microscopy. NPG Asia Mater 8:e327

Gardel M, Shin JH, MacKintosh F, Mahadevan L, Matsudaira P, Weitz D (2004) Elastic behavior of cross-linked and bundled actin networks. Science 304:1301-1305

Gavara N, Chadwick RS (2012) Determination of the elastic moduli of thin samples and adherent cells using conical atomic force microscope tips. Nat Nanotechnol 7:733-736

Geitmann A, Ortega JKE (2009) Mechanics and modeling of plant cell growth. Trends Plant Sci 14:467-478

Hansma HG, Kim KJ, Laney DE, Garcia RA, Argaman M, Allen MJ, Parsons SM (1997) Properties of biomolecules measured from atomic force microscope images:a review. J Struct Biol 119:99-108

Le S, Hu X, Yao M, Chen H, Yu M, Xu X, Nakazawa N, Margadant FM, Sheetz MP, Yan J (2017) Mechanotransmission and mechanosensing of human alpha-Actinin 1. Cell Rep 21:2714-2723

Liu H, Wen J, Xiao Y, Liu J, Hopyan S, Radisic M, Simmons CA, Sun Y (2014) In Situ mechanical characterization of the cell nucleus by atomic force microscopy. ACS Nano 8:3821-3828

Livne A, Bouchbinder E, Geiger B (2014) Cell reorientation under cyclic stretching. Nat Commun 5:3938

Long M, Sato M, Lim CT, Wu J, Adachi T, Inoue Y (2011) Advances in experiments and modeling in micro- and nanobiomechanics:a mini review. Cell Mol Bioeng 4:327-339

Maivald P, Butt HJ, Gould SA, Prater CB, Drake B, Gurley JA, Elings VB, Hansma PK (1991) Using force modulation to image surface elasticities with the atomic force microscope. Nanotechnology 2:103-106

Marshall BT, Long M, Piper JW, Yago T, McEver RP, Zhu C (2003) Direct observation of catch bonds involving cell-adhesion molecules. Nature 423:190-193

Martinelli R, Zeiger AS, Whitfield M, Sciuto TE, Dvorak A, Van Vliet KJ, Greenwood J, Carman CV (2014) Probing the biomechanical contribution of the endothelium to lymphocyte migration:diapedesis by the path of least resistance. J Cell Sci 127:3720-3734

Ohashi T, Ishii Y, Ishikawa Y, Matsumoto T, Sato M (2002) Experimental and numerical analyses of local mechanical properties measured by atomic force microscopy for sheared endothelial cells. Bio-med Mat Eng 12:319-327

Pittenger B, Slade A (2013) Performing quantitative nanomechanical AFM measurements on live cells. Microsc Today 21:12-17

Qi J, Wu B, Feng S, Lü S, Guan C, Zhang X, Qiu D, Hu Y, Zhou Y, Li C, Long M, Jiao YL (2017) Mechanical regulation of organ asymmetry in leaves. Nat Plants 3:724

Radmacher M, Fritz M, Cleveland JP, Walters DA, Hansma PK (1994) Imaging adhesion forces and elasticity of lysozyme adsorbed on mica with the atomic force microscope. Langmuir 10:3809-3814

Rawlett AM, Hopson TJ, Nagahara LA, Tsui RK, Ramachandran GK, Lindsay SM (2002) Electrical measurements of a dithiolated electronic molecule via conducting atomic force microscopy. Appl Phys Lett 81:3043-3045

Rodriguez ML, McGarry PJ, Sniadecki NJ (2013) Review on cell mechanics:experimental and modeling approaches. Appl Mech Rev 65:060801-1-41

Roduit C, van der Goot FG, De Los Rios P, Yersin A, Steiner P, Dietler G, Catsicas S, Lafont F, Kasas S (2008) Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains. Biophys J 94:1521-1532

Schaefer A, Hordijk PL (2015) Cell-stiffness-induced mechanosignaling-a key driver of leukocyte transendothelial migration. J Cell Sci 128:2221-2230

Schillers H, Medalsy I, Hu S, Slade AL, Shaw JE (2016) PeakForce Tapping resolves individual microvilli on living cells. J Mol Recognit 29:95-101

Schmoller K, Fernandez P, Arevalo R, Blair D, Bausch A (2010) Cyclic hardening in bundled actin networks. Nat Commun 1:134

Sneddon IN (1965) The relation between load and penetration in the axisymmetric Boussinesq problem for a punch of arbitrary profile. Int J Eng Sci 3:47-57

Sugimoto Y, Pou P, Abe M, Jelinek P, Pérez R, Morita S, Custance Ó(2007) Chemical identification of individual surface atoms by atomic force microscopy. Nature 446:64-67

Vargas-Pinto R, Gong H, Vahabikashi A, Johnson M (2013) The effect of the endothelial cell cortex on atomic force microscopy measurements. Biophys J 105:300-309

Wang J, Lü D, Mao D, Long M (2014) Mechanomics:an emerging field between biology and biomechanics. Protein Cell 5:518-531

Xu Y, Huang D, Lu S, Zhang Y, Long M (2019) Mechanical features of endothelium regulate cell adhesive molecule-induced calcium response in neutrophils. APL Bioeng 3:016104

Zhong Q, Inniss D, Kjoller K, Elings VB (1993) Fractured polymer/silica fiber surface studied by tapping mode atomic force microscopy. Surf Sci Lett 290:L688-L692

Supplements(0)

Relative Articles

Cited By

Proportional views