Super-resolution microscopy: successful applications in centrosome study and beyond

Jingyan Fu; Chuanmao Zhang

doi:10.1007/s41048-019-00101-x

Volume 5 Issue 5-6

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Jingyan Fu, Chuanmao Zhang. Super-resolution microscopy: successful applications in centrosome study and beyond[J]. Biophysics Reports, 2019, 5(5-6): 235-243. doi: 10.1007/s41048-019-00101-x

Citation:

Jingyan Fu, Chuanmao Zhang. Super-resolution microscopy: successful applications in centrosome study and beyond[J]. Biophysics Reports, 2019, 5(5-6): 235-243. doi: 10.1007/s41048-019-00101-x

Jingyan Fu, Chuanmao Zhang. Super-resolution microscopy: successful applications in centrosome study and beyond[J]. Biophysics Reports, 2019, 5(5-6): 235-243. doi: 10.1007/s41048-019-00101-x

Citation:

Jingyan Fu, Chuanmao Zhang. Super-resolution microscopy: successful applications in centrosome study and beyond[J]. Biophysics Reports, 2019, 5(5-6): 235-243. doi: 10.1007/s41048-019-00101-x

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Super-resolution microscopy: successful applications in centrosome study and beyond

doi: 10.1007/s41048-019-00101-x

Jingyan Fu^1
,,
Chuanmao Zhang²

1 State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
2 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China

Funds: Jingyan Fu, Chuanmao Zhang

Received Date: 16 August 2019
Publish Date: 31 December 2019

Abstract

Abstract

Centrosome is the main microtubule-organizing center in most animal cells. Its core structure, centriole, also assembles cilia and flagella that have important sensing and motility functions. Centrosome has long been recognized as a highly conserved organelle in eukaryotic species. Through electron microscopy, its ultrastructure was revealed to contain a beautiful nine-symmetrical core 60 years ago, yet its molecular basis has only been unraveled in the past two decades. The emergence of super-resolution microscopy allows us to explore the insides of a centrosome, which is smaller than the diffraction limit of light. Super-resolution microscopy also enables the compartmentation of centrosome proteins into different zones and the identification of their molecular interactions and functions. This paper compiles the centrosome architecture knowledge that has been revealed in recent years and highlights the power of several super-resolution techniques.
- Centrosome,
- Super-resolution microscopy,
- SIM,
- STED,
- SMLM

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References(79)

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