Regulation of intracellular Ca<sup>2+</sup>/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation

Fen Hu; Yali Zhao; Zhenhai Hui; Fulin Xing; Jianyu Yang; Imshik Lee; Xinzheng Zhang; Leiting Pan; Jingjun Xu

doi:10.1007/s41048-019-00100-y

Volume 5 Issue 5-6

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Article Navigation > Biophysics Reports > 2019 > 5(5-6): 254-263

Fen Hu, Yali Zhao, Zhenhai Hui, Fulin Xing, Jianyu Yang, Imshik Lee, Xinzheng Zhang, Leiting Pan, Jingjun Xu. Regulation of intracellular Ca2+/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation[J]. Biophysics Reports, 2019, 5(5-6): 254-263. doi: 10.1007/s41048-019-00100-y

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Fen Hu, Yali Zhao, Zhenhai Hui, Fulin Xing, Jianyu Yang, Imshik Lee, Xinzheng Zhang, Leiting Pan, Jingjun Xu. Regulation of intracellular Ca²⁺/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation[J]. Biophysics Reports, 2019, 5(5-6): 254-263. doi: 10.1007/s41048-019-00100-y

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Regulation of intracellular Ca²⁺/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation

doi: 10.1007/s41048-019-00100-y

1 The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, China
2 Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, Shanxi, China

Funds: Leiting Pan

Received Date: 10 June 2019
Publish Date: 31 December 2019

Abstract

Abstract

Bone constantly remodels between resorption by osteoclasts and formation by osteoblasts; therefore the functions of osteoblasts are pivotal for maintaining homeostasis of bone mass. Transient receptor potential vanilloid 4 (TRPV4), a type of mechanosensitive channel, has been reported to be a key regulator in bone remodeling. However, the relationship between TRPV4 and osteoblast function remains largely elusive. Only little is known about the spatial distribution change of TRPV4 during osteoblastic differentiation and related signal events. Based on three-dimensional super-resolution microscopy, our results clearly showed a different distribution of TRPV4 in undifferentiated and differentiated osteoblasts, which reflected the plasma membrane translocation of TRPV4 along with prolonged differentiation. GSK1016790A (GSK101), the most potent agonist of TRPV4, triggered rapid calcium entry and calmodulin-dependent protein kinase II (CaMKII) phosphorylation via TRPV4 activation in a differentiation-dependent manner, indicating that the abundance of TRPV4 at the cell surface resulting from differentiation may be related to the modulation of Ca²⁺ response and CaMKII activity. These data provide compelling evidences for the plasma membrane translocation of TRPV4 during osteoblastic differentiation as well as demonstrate the regulation of downstream Ca²⁺/CaMKII signaling.
- Osteoblasts,
- Differentiation,
- TRPV4 channel,
- Plasma membrane translocation,
- Super-resolution microscopy,
- Ca²⁺ Signaling

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References(43)

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