Raman spectra of the GFP-like fluorescent proteins

Ye Yuan; Dianbing Wang; Jibin Zhang; Ji Liu; Jian Chen; Xian-En Zhang

doi:10.1007/s41048-018-0072-0

Volume 4 Issue 5

Nov. 2018

Turn off MathJax

Article Contents

Article Navigation > Biophysics Reports > 2018 > 4(5): 265-272

Ye Yuan, Dianbing Wang, Jibin Zhang, Ji Liu, Jian Chen, Xian-En Zhang. Raman spectra of the GFP-like fluorescent proteins[J]. Biophysics Reports, 2018, 4(5): 265-272. doi: 10.1007/s41048-018-0072-0

Citation:

Ye Yuan, Dianbing Wang, Jibin Zhang, Ji Liu, Jian Chen, Xian-En Zhang. Raman spectra of the GFP-like fluorescent proteins[J]. Biophysics Reports, 2018, 4(5): 265-272. doi: 10.1007/s41048-018-0072-0

Ye Yuan, Dianbing Wang, Jibin Zhang, Ji Liu, Jian Chen, Xian-En Zhang. Raman spectra of the GFP-like fluorescent proteins[J]. Biophysics Reports, 2018, 4(5): 265-272. doi: 10.1007/s41048-018-0072-0

Citation:

Ye Yuan, Dianbing Wang, Jibin Zhang, Ji Liu, Jian Chen, Xian-En Zhang. Raman spectra of the GFP-like fluorescent proteins[J]. Biophysics Reports, 2018, 4(5): 265-272. doi: 10.1007/s41048-018-0072-0

PDF (998 KB)

Raman spectra of the GFP-like fluorescent proteins

doi: 10.1007/s41048-018-0072-0

1 National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2 College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
3 College of Life Science, Hubei University, Wuhan 430070, China

Funds: This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences (XDPB0305, CAS).

More Information

Corresponding author: Xian-En Zhang
Received Date: 17 July 2018
Publish Date: 02 November 2018

Abstract

Abstract

The objective of the study was to elucidate optical characteristics of the chromophore structures of fluorescent proteins. Raman spectra of commonly used GFP-like fluorescent proteins (FPs) with diverse emission wavelengths (green, yellow, cyan and red), including the enhanced homogenous FPs EGFP, EYFP, and ECFP (from jellyfish) as well as mNeptune (from sea anemone) were measured. High-quality Raman spectra were obtained and many marker bands for the chromophore of the FPs were identified via assignment of Raman spectra bands. We report the presence of a positive linear correlation between the Raman band shift of C₅=C₆ and the excitation energy of FPs, demonstrated by plotting absorption maxima (cm^-1) against the position of the Raman band C₅=C₆ in EGFP, ECFP, EYFP, the anionic chromophore and the neutral chromophore. This study revealed new Raman features in the chromophores of the observed FPs, and may contribute to a deeper understanding of the optical properties of FPs.
- Raman spectra,
- Fluorescent protein,
- Chromophore

FullText(HTML)

References(13)

References

Arpino JA, Rizkallah PJ, Jones DD (2012) Crystal structure of enhanced green fluorescent protein to 1.35 Å resolution reveals alternative conformations for Glu222. PLoS ONE 7(10):e47132

Bell AF, He X, Wachter RM, Tonge PJ (2000) Probing the ground state structure of the green fluorescent protein chromophore using Raman spectroscopy. Biochemistry 39:4423-4431

Bunaciu AA, Aboulenein HY, Hoang VD (2015) Raman spectroscopy for protein analysis. Appl Spectrosc Rev 50:377-386

Carey P (1982) Biochemical applications of raman and resonance raman spectroscopes. In:Chapter 4 protein conformation from raman and resonance raman sptecta, pp 71-98

Fang C, Frontiera RR, Tran R, Mathies RA (2009) Mapping GFP structure evolution during proton transfer with femtosecond Raman spectroscopy. Nature 462:200-204

Higashino A, Mizuno M, Mizutani Y (2016) Chromophore structure of photochromic fluorescent protein Dronpa:acid-base equilibrium of two cis configurations. J Phys Chem B 120:3353

Lelimousin M, Noirclerc-Savoye M, Lazareno-Saez C, Paetzold B, Le VS, Chazal R, Macheboeuf P, Field MJ, Bourgeois D, Royant A (2009) Intrinsic dynamics in ECFP and Cerulean control fluorescence quantum yield. Biochemistry 48:10038-10046

Loos Davey C, Habuchi Satoshi, Flors Cristina, Hotta Junichi, Jörg Wiedenmann G, Nienhaus Ulrich, Hofkens Johan (2006) Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor:is cis-trans isomerization involved? J Am Chem Soc 128:6270-6271

Patterson G, Day RN, Piston D (2001) Fluorescent protein spectra.J Cell Sci 114:837-838

Tu AT (1982) Raman spectroscopy in biology:principles and applications. Wiley, New York Tuma R (2005) Raman spectroscopy of proteins:from peptides to large assemblies. J Raman Spectrosc 36:307-319

Voityuk AA, Michel-Beyerle ME, Rösch N (1998) Quantum chemical modeling of structure and absorption spectra of the chromophore in green fluorescent proteins. Chem Phys 231(1):13-25

Wachter RM, Elsliger MA, Kallio K, Hanson GT, Remington SJ (1998) Structural basis of spectral shifts in the yellowemission variants of green fluorescent protein.Structure 6:1267-1277

Xu Y (2005) Raman spectroscopy in application of structure biology. In:Chapter 2 proteins. Chemical Industry Press, Beijing, pp 11-26

Supplements(0)

Relative Articles

Cited By

Proportional views