Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers

Xue Xiao; Liping Dong; Yi-Zhou Wang; Peng-Ye Wang; Ming Li; Guohong Li; Ping Chen; Wei Li

doi:10.1007/s41048-018-0064-0

Volume 4 Issue 4

Aug. 2018

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Article Navigation > Biophysics Reports > 2018 > 4(4): 222-232

Xue Xiao, Liping Dong, Yi-Zhou Wang, Peng-Ye Wang, Ming Li, Guohong Li, Ping Chen, Wei Li. Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers[J]. Biophysics Reports, 2018, 4(4): 222-232. doi: 10.1007/s41048-018-0064-0

Citation:

Xue Xiao, Liping Dong, Yi-Zhou Wang, Peng-Ye Wang, Ming Li, Guohong Li, Ping Chen, Wei Li. Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers[J]. Biophysics Reports, 2018, 4(4): 222-232. doi: 10.1007/s41048-018-0064-0

Citation:

Xue Xiao, Liping Dong, Yi-Zhou Wang, Peng-Ye Wang, Ming Li, Guohong Li, Ping Chen, Wei Li. Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers[J]. Biophysics Reports, 2018, 4(4): 222-232. doi: 10.1007/s41048-018-0064-0

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Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers

doi: 10.1007/s41048-018-0064-0

Xue Xiao^1,3,
Liping Dong^2,3,
Yi-Zhou Wang^1,3,
Peng-Ye Wang^1,3,
Ming Li^1,3,
Guohong Li^2,3,
Ping Chen^2
,,
Wei Li¹

1 National Laboratory for Condensed Matter Physics and Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
2 National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
3 University of Chinese Academy of Sciences, Beijing 100049, China

Funds: This work was supported by Grants from the National Natural Science Foundation of China (11474346, 31471218, 31630041, 31525013, 31521002 and 61275192), the Key Research Program of Frontier Sciences, CAS (QYZDB-SSWSLH045, QYZDY-SSW-SMC020), the Youth Innovation Promotion Association CAS (2015071), the Ministry of Science and Technology of China (2017YFA0504200, 2015CB856200), (HHMI International Research Scholar Grant (55008737),and the National Key Research and Development Program (2016YFA0301500).

More Information

Corresponding author: Ping Chen, Wei Li
Received Date: 26 June 2018
Publish Date: 01 August 2018

Abstract

Abstract

The accessibility of genomic DNA, as a key determinant of gene-related processes, is dependent on the packing density and structural dynamics of chromatin fiber. However, due to the highly dynamic and heterogeneous properties of chromatin fiber, it is technically challenging to study these properties of chromatin. Here, we report a strategy for dissecting the dynamics of chromatin fibers based on singlemolecule magnetic tweezers. Using magnetic tweezers, we can manipulate the chromatin fiber and trace its extension during the folding and unfolding process under tension to investigate the dynamic structural transitions at single-molecule level. The highly accurate and reliable in vitro single-molecule strategy provides a new research platform to dissect the structural dynamics of chromatin fiber and its regulation by different epigenetic factors during gene expression.
- Magnetic tweezers,
- Chromatin fiber,
- Dynamics,
- Single molecule

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References(54)

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