Identification of small ORF-encoded peptides in mouse serum

Yaqin Deng; Adekunle Toyin Bamigbade; Mirza Ahmed Hammad; Shimeng Xu; Pingsheng Liu

doi:10.1007/s41048-018-0048-0

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Yaqin Deng, Adekunle Toyin Bamigbade, Mirza Ahmed Hammad, Shimeng Xu, Pingsheng Liu. Identification of small ORF-encoded peptides in mouse serum. Biophysics Reports, 2018, 4(1): 39-49. doi: 10.1007/s41048-018-0048-0

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Yaqin Deng, Adekunle Toyin Bamigbade, Mirza Ahmed Hammad, Shimeng Xu, Pingsheng Liu. Identification of small ORF-encoded peptides in mouse serum. Biophysics Reports, 2018, 4(1): 39-49. doi: 10.1007/s41048-018-0048-0

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Identification of small ORF-encoded peptides in mouse serum

doi: 10.1007/s41048-018-0048-0

1 National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2 University of Chinese Academy of Sciences, Beijing 100049, China

Funds: This work was supported by the Ministry of Science and Technology of China (2016YFA0500100), National Natural Science Foundation of China (U1402225, 31571388, 31671402, 31671233, 31701018, and 81471082). This work was also supported by the "Personalized Medicines-Molecular Signature-based Drug Discovery and Development," Strategic Priority Research Program of the Chinese Academy of Sciences (XDA12030201). This work was also supported by the CAS-Croucher Joint Laboratory Project (CAS16SC01). Bamigbade Adekunle Toyin sincerely acknowledged the CAS-TWAS President's Fellowship.

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Corresponding author: Shimeng Xu,xushimeng@ibp.ac.cn;Pingsheng Liu,pliu@ibp.ac.cn
Received Date: 13 September 2017
Rev Recd Date: 05 December 2017
Publish Date: 28 February 2018

Abstract

Abstract

Identification of the coding elements in the genome is fundamental to interpret the development of living systems and species diversity. Small peptides (length <100 amino acids) have played an important role in regulating the biological metabolism, but their identification has been limited by their size and abundance. Serum is the most important body fluid and is full of small peptides. In this study, we have established a small ORF-encoded peptides (SEPs) database from mouse GENCODE release. This database provides about half a million putative translated SEPs in mouse. We also extract serum proteins from wild type and ob/ob mice, and collect the low molecular weight proteins for mass spectrometric analysis. More than 50 novel SEPs have been discovered. Several SEPs are further verified by biochemical method with newly raised antibodies. These novel SEPs enhance the knowledge about the complexity of serum and provide new clues for the annotation and functional analysis of genes, especially the noncoding elements in the genome.
- Small ORF-encoded peptides (SEPs),
- Serum,
- Database,
- Mass spectrometric analysis,
- ob/ob mice

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References(32)

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