Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria <em>in situ</em>

Shengliu Wang; Shuoguo Li; Gang Ji; Xiaojun Huang; Fei Sun

doi:10.1007/s41048-017-0035-x

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Shengliu Wang, Shuoguo Li, Gang Ji, Xiaojun Huang, Fei Sun. Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ. Biophysics Reports, 2017, 3(1-3): 8-16. doi: 10.1007/s41048-017-0035-x

Citation:

Shengliu Wang, Shuoguo Li, Gang Ji, Xiaojun Huang, Fei Sun. Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ. Biophysics Reports, 2017, 3(1-3): 8-16. doi: 10.1007/s41048-017-0035-x

Citation:

Shengliu Wang, Shuoguo Li, Gang Ji, Xiaojun Huang, Fei Sun. Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ. Biophysics Reports, 2017, 3(1-3): 8-16. doi: 10.1007/s41048-017-0035-x

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Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ

doi: 10.1007/s41048-017-0035-x

1.
National Key Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2. Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
3 University of Chinese Academy of Sciences, Beijing, China

Funds: This work was supported by grants from the Strategic Priority Research Program of Chinese Academy of Sciences (XDB08030202) and the National Basic Research Program ("973" Program) of the Ministry of Science and Technology of China (2014CB910700).

More Information

Corresponding author: Fei Sun,feisun@ibp.ac.cn
Received Date: 19 August 2016
Rev Recd Date: 28 December 2016
Publish Date: 31 March 2017

Abstract

Abstract

Correlative cryo-fluorescence and cryo-electron microscopy (cryo-CLEM) system has been fast becoming a powerful technique with the advantage to allow the fluorescent labeling and direct visualization of the close-to-physiologic ultrastructure in cells at the same time, offering unique insights into the ultrastructure with specific cellular function. There have been various engineered ways to achieve cryo-CLEM including the commercial FEI iCorr system that integrates fluorescence microscope into the column of transmission electron microscope. In this study, we applied the approach of the cryo-CLEMbased iCorr to image the syntaphilin-immobilized neuronal mitochondria in situ to test the performance of the FEI iCorr system and determine its correlation accuracy. Our study revealed the various morphologies of syntaphilin-immobilized neuronal mitochondria that interact with microtubules and suggested that the cryo-CLEM procedure by the FEI iCorr system is suitable with a half micron-meter correlation accuracy to study the cellular organelles that have a discrete distribution and large size, e.g. mitochondrion, Golgi complex, lysosome, etc.
- Correlative cryo-light and electron microscopy,
- iCorr,
- Mitochondria,
- Primary hippocampal neuron cell,
- Syntaphilin

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References(33)

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