Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing

Zhenbo Cao; Neil J. Bulleid

doi:10.1007/s41048-015-0007-y

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Zhenbo Cao, Neil J. Bulleid. Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing. Biophysics Reports, 2015, 1(1): 14-17. doi: 10.1007/s41048-015-0007-y

Citation:

Zhenbo Cao, Neil J. Bulleid. Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing. Biophysics Reports, 2015, 1(1): 14-17. doi: 10.1007/s41048-015-0007-y

Zhenbo Cao, Neil J. Bulleid. Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing. Biophysics Reports, 2015, 1(1): 14-17. doi: 10.1007/s41048-015-0007-y

Citation:

Zhenbo Cao, Neil J. Bulleid. Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing. Biophysics Reports, 2015, 1(1): 14-17. doi: 10.1007/s41048-015-0007-y

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Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing

doi: 10.1007/s41048-015-0007-y

Zhenbo Cao,
Neil J. Bulleid^,

Institute of Molecular, Cellular and Systems Biology, College of Medical Veterinary and Life Sciences, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK

More Information

Corresponding author: Neil J. Bulleid
Received Date: 02 March 2014
Rev Recd Date: 30 March 2015
Publish Date: 31 August 2015

Abstract

Abstract

The activity of typical 2-cys peroxiredoxin (Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously. This assay only requires standard equipment and can compare different samples on the same gel. It requires much less time than conventional 2D gels and gives more information than Western blotting with an antibody specific for hyperoxidized peroxiredoxin. This method could also be used to monitor protein modification with a charge difference such as phosphorylation.
- Hyperoxidation,
- Peroxiredoxin,
- Isoelectric focusing

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References

参考文献

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