A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example

Lin Cao; Shouhai Zhu; Yaxin Wang; Zhiyong Lou; Yuna Sun

doi:10.1007/s41048-015-0006-z

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Lin Cao, Shouhai Zhu, Yaxin Wang, Zhiyong Lou, Yuna Sun. A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example. Biophysics Reports, 2015, 1(2): 81-89. doi: 10.1007/s41048-015-0006-z

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Lin Cao, Shouhai Zhu, Yaxin Wang, Zhiyong Lou, Yuna Sun. A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example. Biophysics Reports, 2015, 1(2): 81-89. doi: 10.1007/s41048-015-0006-z

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A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example

doi: 10.1007/s41048-015-0006-z

1 College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
2 National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China
3 Laboratory of Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China

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Corresponding author: Yuna Sun
Received Date: 15 November 2014
Rev Recd Date: 02 March 2015
Publish Date: 03 October 2015

Abstract

Abstract

The prevalence of chronic viral infectious diseases, the emergence and re-emergence of new viral infections, and in particular, resistance to currently used antiviral drugs have led to increased demand for new antiviral strategies and reagents. Increased understanding of the molecular mechanisms of viral infection has provided great potential for the discovery of new antiviral agents that target viral proteins or host factors. In this work, we introduce a comprehensive system using enteroviruses 71 (EV71) as an example for leading compound discovery to develop new antiviral.
- Virus,
- Antiviral,
- Entry,
- Replication,
- Assay

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参考文献

Chen C, Wang Y, Shan C, Sun Y, Xu P, Zhou H, Yang C, Shi PY, Rao Z, Zhang B, Lou Z (2013) Crystal structure of enterovirus 71 RNA-dependent RNA polymerase complexed with Its protein primer VPg: implication for a trans mechanism of VPg uridylylation. J Virol 87:5755-5768

Lin YC, Wu CN, Shih SR, Ho MS (2002) Characterization of a vero cell-adapted virulent strain of enterovirus 71 suitable for use as a vaccine candidate. Vaccine 20:2485-2493

Lou Z, Sun Y, Rao Z (2013) Current progress in antiviral strategies. Trends Pharmacol Sci. doi: 10.1016/j.tips.2013.1011.1006

Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3:1101-1108

Sun Y, Wang Y, Shan C, Chen C, Xu P, Song M, Zhou H, Yang C, Xu W, Shi PY, Zhang B, Lou Z (2012) Enterovirus 71 VPg uridylation uses a two-molecular mechanism of 3D polymerase. J Virol 86:13662-13671

Zhou H, Sun Y, Guo Y, Lou Z (2013) Structural perspective on the formation of ribonucleoprotein complex in negative-sense single-stranded RNA viruses. Trends Microbiol 21:475-484

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