Phosphorylation and function of DGAT1 in skeletal muscle cells

Jinhai Yu; Yiran Li; Fei Zou; Shimeng Xu; Pingsheng Liu

doi:10.1007/s41048-015-0004-1

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Jinhai Yu, Yiran Li, Fei Zou, Shimeng Xu, Pingsheng Liu. Phosphorylation and function of DGAT1 in skeletal muscle cells. Biophysics Reports, 2015, 1(1): 41-50. doi: 10.1007/s41048-015-0004-1

Citation:

Jinhai Yu, Yiran Li, Fei Zou, Shimeng Xu, Pingsheng Liu. Phosphorylation and function of DGAT1 in skeletal muscle cells. Biophysics Reports, 2015, 1(1): 41-50. doi: 10.1007/s41048-015-0004-1

Jinhai Yu, Yiran Li, Fei Zou, Shimeng Xu, Pingsheng Liu. Phosphorylation and function of DGAT1 in skeletal muscle cells. Biophysics Reports, 2015, 1(1): 41-50. doi: 10.1007/s41048-015-0004-1

Citation:

Jinhai Yu, Yiran Li, Fei Zou, Shimeng Xu, Pingsheng Liu. Phosphorylation and function of DGAT1 in skeletal muscle cells. Biophysics Reports, 2015, 1(1): 41-50. doi: 10.1007/s41048-015-0004-1

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Phosphorylation and function of DGAT1 in skeletal muscle cells

doi: 10.1007/s41048-015-0004-1

Jinhai Yu^1,2,
Yiran Li^1,3,
Fei Zou^4,5,
Shimeng Xu^{1,2
,
,},
Pingsheng Liu^1
,

1 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
3 Department of Biological Science and Biotechnology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
4 Graduate School of Anhui Medical University, Hefei 230032, China
5 General Hospital of Air Force, Beijing 100142, China

More Information

Corresponding author: Shimeng Xu, Pingsheng Liu
Received Date: 08 January 2015
Rev Recd Date: 27 April 2015
Publish Date: 31 August 2015

Abstract

Abstract

Aberrant intramuscular triacylglycerol (TAG) storage in human skeletal muscle is closely related to insulin insensitivity. Excessive lipid storage can induce insulin resistance of skeletal muscle, and under severe conditions, lead to type 2 diabetes. The balance of interconversion between diacylglycerol and TAG greatly influences lipid storage and utilization. Diacylglycerol O-acyltransferase 1 (DGAT1) plays a key role in this process, but its activation and phosphorylation requires further dissection. In this study, 12 putative conserved phosphorylation sites of DGAT1 were identified by examining amino acid conservation of DGAT1 in different species. Another 12 putative phosphorylation sites were also found based on bioinformatics predictions and previous reports. Meanwhile, several phosphorylation sites of DGAT1 were verified by phosphorylation mass spectrometry analysis of purified DGAT1 from mouse myoblast C2C12 cells. Using single point mutations, a regulatory role of 3 putative phosphorylation sites was dissected. Finally, using truncation mutations, a potential domain of DGAT1 that was involved in the regulation of enzymatic activity was revealed. This study provides useful information for further understanding DGAT1 activity regulation.
- DGAT1,
- Phosphorylation,
- Single point mutation,
- Enzymatic activity

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References(38)

References

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