Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis

Xiangdong Xue; Yuanyuan Zhao; Xu Zhang; Chunqiu Zhang; Anil Kumar; Xiaoning Zhang; Guozhang Zou; Paul C. Wang; Jinchao Zhang; Xing-Jie Liang

doi:10.1007/s41048-015-0002-3

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Xiangdong Xue, Yuanyuan Zhao, Xu Zhang, Chunqiu Zhang, Anil Kumar, Xiaoning Zhang, Guozhang Zou, Paul C. Wang, Jinchao Zhang, Xing-Jie Liang. Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis. Biophysics Reports, 2015, 1(2): 61-70. doi: 10.1007/s41048-015-0002-3

Citation:

Xiangdong Xue, Yuanyuan Zhao, Xu Zhang, Chunqiu Zhang, Anil Kumar, Xiaoning Zhang, Guozhang Zou, Paul C. Wang, Jinchao Zhang, Xing-Jie Liang. Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis. Biophysics Reports, 2015, 1(2): 61-70. doi: 10.1007/s41048-015-0002-3

Citation:

Xiangdong Xue, Yuanyuan Zhao, Xu Zhang, Chunqiu Zhang, Anil Kumar, Xiaoning Zhang, Guozhang Zou, Paul C. Wang, Jinchao Zhang, Xing-Jie Liang. Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis. Biophysics Reports, 2015, 1(2): 61-70. doi: 10.1007/s41048-015-0002-3

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Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis

doi: 10.1007/s41048-015-0002-3

1 CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China
2 Laboratory of Pharmaceutics, School of Medicine, Tsinghua University, Beijing 100084, China
3 College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding 071002, China
4 Fu Jen Catholic University, Taipei 24205, China

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Corresponding author: Guozhang Zou, Xing-Jie Liang
Received Date: 18 January 2015
Rev Recd Date: 01 April 2015
Publish Date: 03 October 2015

Abstract

Abstract

In this work, 2-(2-aminoethoxy) ethanolblocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molecules, and the 2-(2-aminoethoxy) ethanol can deplete the nonspecific absorption of peptides and proteins which always coexisted with saccharides. For mass spectrometry analysis, the PMNPs bound saccharides can be directly applied onto the MALDI plate with matrix without removing the PMNPs. By PMNPs, the simple saccharide (glucose) could be detected in pmol level. The complex saccharides can also be reliably purified and analyzed; 16 different Nglycans were successfully identified fromovalbumin, and the high-abundance N-glycans can be detected even when the ovalbumin was in very low concentration (2 μg). In human milk, ten different oligosaccharides were identified, and the lactose can still be detected when the human milk concentration was low to 0.01 μL.
- Magnetic nanoparticles,
- MALDI-TOF MS,
- Glucose,
- N-Glycans,
- Oligosaccharides

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