2017 Vol. 3, No. 1-3
Cover Story
Correlative cryo-fluorescence and cryo-electron microscopy (cryo-CLEM) system has been fast becoming a powerful technique with the advantage to allow the fluorescent labeling and direct visu-alization of the close-tophysiologic ultrastructure in cells at the same time,offering unique insights into the ultrastructure with specific cellular function.There have been various engineered ways to achieve cryo-CLEM including the commercial FEI iCorr system that integrates fluorescence microscope into the column of transmission electron microscope.In this study,the authors applied the approach of the cryo-CLEM-based iCorr to image the syntaphilinimmobilized neuronal mitochondria in situ to test the performance of the FEI iCorr system and determine its correlation accuracy.This study revealed the various mor-phologies of syntaphilin-immobilized neuronal mitochondria that interact with microtubules and suggested that the cryo- CLEM procedure by the FEI iCorr system is suitable with a half micronmeter correlation accuracy to study the cellular organelles that have a discrete distribution and large size,e.g.mitochondrion,Golgi complex, lysosome,etc.