Detection of ribophagy in yeast and mammals

Yuting Chen; Miao Ye; Zhaojie Liu; Yigang Wang; Cong Yi

doi:10.52601/bpr.2024.240002

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Yuting Chen, Miao Ye, Zhaojie Liu, Yigang Wang, Cong Yi. Detection of ribophagy in yeast and mammals[J]. Biophysics Reports. doi: 10.52601/bpr.2024.240002

Citation:

Yuting Chen, Miao Ye, Zhaojie Liu, Yigang Wang, Cong Yi. Detection of ribophagy in yeast and mammals[J]. Biophysics Reports. doi: 10.52601/bpr.2024.240002

Yuting Chen, Miao Ye, Zhaojie Liu, Yigang Wang, Cong Yi. Detection of ribophagy in yeast and mammals[J]. Biophysics Reports. doi: 10.52601/bpr.2024.240002

Citation:

Yuting Chen, Miao Ye, Zhaojie Liu, Yigang Wang, Cong Yi. Detection of ribophagy in yeast and mammals[J]. Biophysics Reports. doi: 10.52601/bpr.2024.240002

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Detection of ribophagy in yeast and mammals

doi: 10.52601/bpr.2024.240002

Yuting Chen^{2, Y},
Miao Ye^{1, Y},
Zhaojie Liu^{3, Y},
Yigang Wang^{1
,
,},
Cong Yi^{2
,
,}

1.
Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
2.
Department of Biochemistry, and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China
3.
Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 510182, China

More Information

Corresponding author: wangyigang43@163.com (Y. Wang); yiconglab@zju.edu.cn (C. Yi)
Received Date: 08 January 2024
Accepted Date: 01 February 2024

Available Online: 28 March 2024

Abstract

Abstract

Ribophagy, the cellular process wherein ribosomes are selectively self-digested through autophagy, plays a pivotal role in maintaining ribosome turnover. Understanding the molecular regulatory mechanisms governing ribophagy is pivotal to uncover its significance. Consequently, the establishment of methods for detecting ribophagy becomes important. In this protocol, we have optimized, enriched, and advanced existing ribophagy detection techniques, including immunoblotting, fluorescence microscopy, and transmission electron microscopy (TEM), to precisely monitor and quantify ribophagic events. Particularly noteworthy is the introduction of TEM technology for yeast ribophagy detection. In summary, the delineated methods are applicable for detecting ribophagy in both yeast and mammals, laying a solid foundation for further exploring the physiological importance of ribophagy and its potential implications in diverse cellular environments.
- Ribophagy,
- Ribosome turnover,
- Immunoblotting,
- Fluorescence microscopy,
- Transmission electron microscopy,
- Yeast and mammals

Yuting Chen, Miao Ye, Zhaojie Liu, Yigang Wang, and Cong Yi declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by the any of the authors.
Yuting Chen, Miao Ye and Zhaojie Liu contributed equally to this work.

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