Assaying sarcoplasmic reticulum Ca<sup>2+</sup>-leak in mouse atrial myocytes

Fan Xu; Jing-Jing Li; Eric Yang; Yi Zhang; Wenjun Xie

doi:10.52601/bpr.2023.230044

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Fan Xu, Jing-Jing Li, Eric Yang, Yi Zhang, Wenjun Xie. Assaying sarcoplasmic reticulum Ca2+-leak in mouse atrial myocytes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230044

Citation:

Fan Xu, Jing-Jing Li, Eric Yang, Yi Zhang, Wenjun Xie. Assaying sarcoplasmic reticulum Ca²⁺-leak in mouse atrial myocytes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230044

Fan Xu, Jing-Jing Li, Eric Yang, Yi Zhang, Wenjun Xie. Assaying sarcoplasmic reticulum Ca2+-leak in mouse atrial myocytes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230044

Citation:

Fan Xu, Jing-Jing Li, Eric Yang, Yi Zhang, Wenjun Xie. Assaying sarcoplasmic reticulum Ca²⁺-leak in mouse atrial myocytes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230044

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Assaying sarcoplasmic reticulum Ca²⁺-leak in mouse atrial myocytes

doi: 10.52601/bpr.2023.230044

1.
School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710061, China
2.
Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi’an 710061, China
3.
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, and Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China

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Corresponding author: xiewenjun@xjtu.edu.cn (W. Xie)
Received Date: 06 December 2023
Accepted Date: 26 December 2023

Available Online: 26 February 2024

Abstract

Abstract

More and more studies have suggested an essential role of sarcoplasmic reticulum (SR) Ca²⁺ leak of atrial myocytes in atrial diseases such as atrial fibrillation (AF). The increasing interest in atrial Ca²⁺ signaling makes it necessary to develop a more accurate approach for Ca²⁺ measurement in atrial myocytes due to obvious differences between atrial and ventricular Ca²⁺ handling. In the present study, we proposed a new approach for quantifying total SR Ca²⁺ leak in atrial myocytes with confocal line-scan Ca²⁺ images. With a very precious approximation of the histogram of normalized line-scan Ca²⁺ images by using a modified Gaussian distribution, we separated the signal pixel components from noisy pixels and extracted two new dimensionless parameters, F_signals and R_signals, to reflect the summation of signal pixels and their release components, respectively. In the presence of tetracaine blocking SR Ca²⁺ leak, the two parameters were very close to 0, and in atrial myocytes under normal conditions, the two parameters are well positive correlative with Ca²⁺ spark frequency and total signal mass, the two classic readouts for SR Ca²⁺ leak. Consistent with Ca²⁺ Spark readouts, the two parameters quantified a significant increase of SR Ca²⁺ leak in atrial myocytes from mice harboring a leaky type 2 ryanodine receptor mutation (RyR2-R2474S^+/−) compared to the WT group. Collectively, this study proposed a simple and effective approach to quantify SR Ca²⁺ leak in atrial myocytes, which may benefit research on calcium signaling in atrial physiology and diseases.
- Atrial myocyte,
- Sarcoplasmic reticulum,
- Ca²⁺ leak,
- Confocal images,
- Ca²⁺ measurement

Fan Xu, Jing-Jing Li, Eric Yang, Yi Zhang and Wenjun Xie declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.

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References(30)

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