An optimized method of culturing neurons based on polyacrylamide gel

Yongjing Qiao; Jihong Gong; Ziqi Jin; Yiting Tu; Xiaofei Yang

doi:10.52601/bpr.2023.230033

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Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu, Xiaofei Yang. An optimized method of culturing neurons based on polyacrylamide gel[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230033

Citation:

Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu, Xiaofei Yang. An optimized method of culturing neurons based on polyacrylamide gel[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230033

Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu, Xiaofei Yang. An optimized method of culturing neurons based on polyacrylamide gel[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230033

Citation:

Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu, Xiaofei Yang. An optimized method of culturing neurons based on polyacrylamide gel[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230033

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An optimized method of culturing neurons based on polyacrylamide gel

doi: 10.52601/bpr.2023.230033

Yongjing Qiao^{1, Y},
Jihong Gong^{1, Y
,
,},
Ziqi Jin¹,
Yiting Tu¹,
Xiaofei Yang^{1
,
,}

1.
Key Laboratory of Cognitive Science, Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis & Treatment, Laboratory of Membrane Ion Channels and Medicine, College of Biomedical Engineering, South-Central Minzu University, Wuhan 430074, China

More Information

Author Bio:
Yongjing Qiao and Jihong Gong
Corresponding author: sunlittlefly@hotmail.com (X. Yang); jihonggong@foxmail.com (J. Gong)
Received Date: 02 November 2023
Accepted Date: 12 January 2024

Available Online: 05 March 2024

Abstract

Abstract

Substrate stiffness is a microenvironment with a certain stiffness constructed by the extracellular matrix and adjacent cells, which plays an important role in the growth and development of cells and tissue formation. Studies have indicated that the stiffness of the brain is about 0.1–1 kPa. The physiological and pathological processes of the nervous system are mediated by the substrate stiffness that the neurons suffer. However, how substrate stiffness regulates these processes remains to be studied. Culturing neurons on substrates with different stiffness in vitro is one of the best methods to study the role of stiffness in regulating neuronal development and activity. In this study, by changing the preparation time and the activation time of polyacrylamide gel, we provide an improved method that achieves a low toxic substrate environment for better primary neuron adhesion and development. Hope that this method is convenient for those studying the role of substrate stiffness in neurons.
- Polyacrylamide gel,
- Neuron culture,
- Cell adhesion,
- Neural cell development

Yongjing Qiao, Jihong Gong, Ziqi Jin, Yiting Tu and Xiaofei Yang declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.
Yongjing Qiao and Jihong Gong contributed equally to this work.

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References(18)

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