Seeing is believing: observation of migrasomes

Yuwei Huang; Li Yu

doi:10.52601/bpr.2023.230024

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Yuwei Huang, Li Yu. Seeing is believing: observation of migrasomes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230024

Citation:

Yuwei Huang, Li Yu. Seeing is believing: observation of migrasomes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230024

Yuwei Huang, Li Yu. Seeing is believing: observation of migrasomes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230024

Citation:

Yuwei Huang, Li Yu. Seeing is believing: observation of migrasomes[J]. Biophysics Reports. doi: 10.52601/bpr.2023.230024

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Seeing is believing: observation of migrasomes

doi: 10.52601/bpr.2023.230024

Yuwei Huang¹,
Li Yu^{2
,
,}

1.
School of Basic Medical Sciences, Translational Medicine Institute, Xi’an Jiaotong University, Xi’an 710049, China
2.
State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China

More Information

Corresponding author: liyulab@mail.tsinghua.edu.cn (L. Yu)
Received Date: 22 October 2023
Accepted Date: 29 December 2023

Available Online: 05 March 2024

Abstract

Abstract

Migrasomes are a novel type of cell organelle that form on the retraction fibers at the rear of migrating cells. In recent years, numerous studies have unveiled the mechanisms of migrasome formation and have highlighted significant roles of migrasomes in both physiological and pathological processes. Building upon the strategies outlined in published works and our own research experiences, we have compiled a comprehensive set of protocols for observing migrasomes. These step-by-step instructions encompass various aspects such as cell culture, labeling, imaging, in vitro reconstitution, and statistical analysis. We believe that these protocols serve as a valuable resource for researchers exploring migrasome biology.
- Migrasome,
- Labeling,
- Imaging,
- In vitro reconstitution

Yuwei Huang and Li Yu declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.

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References(19)

References

Dharan R, Goren S, Cheppali SK, Shendrik P, Brand G, Vaknin A, Yu L, Kozlov MM, Sorkin R (2022) Transmembrane proteins tetraspanin 4 and CD9 sense membrane curvature. Proc Natl Acad Sci USA 119(43): e2208993119. https://doi.org/10.1073/pnas.2208993119

Dharan R, Huang Y, Cheppali SK, Goren S, Shendrik P, Wang W, Qiao J, Kozlov MM, Yu L, Sorkin R (2023) Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes. Nat Commun 14(1): 1037. https://doi.org/10.1038/s41467-023-36596-9

Ding T, Ji J, Zhang W, Liu Y, Liu B, Han Y, Chen C, Yu L (2023) The phosphatidylinositol (4, 5)-bisphosphate-Rab35 axis regulates migrasome formation. Cell Res 33(8): 617−627 doi: 10.1038/s41422-023-00811-5

Fan C, Shi X, Zhao K, Wang L, Shi K, Liu YJ, Li H, Ji B, Jiu Y (2022) Cell migration orchestrates migrasome formation by shaping retraction fibers. J Cell Biol 221(4): e202109168. https://doi.org/10.1083/jcb.202109168

Hu M, Li T, Ma X, Liu S, Li C, Huang Z, Lin Y, Wu R, Wang S, Lu D, Lu T, Men X, Shen S, Huang H, Liu Y, Song K, Jian B, Jiang Y, Qiu W, Liu Q, Lu Z, Cai W (2023) Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model. Nat Commun 14(1): 3945. https://doi.org/10.1038/s41467-023-39693-x

Huang Y, Zucker B, Zhang S, Elias S, Zhu Y, Chen H, Ding T, Li Y, Sun Y, Lou J, Kozlov MM, Yu L (2019) Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains. Nat Cell Biol 21(8): 991−1002 doi: 10.1038/s41556-019-0367-5

Jiang D, Jiang Z, Lu D, Wang X, Liang H, Zhang J, Meng Y, Li Y, Wu D, Huang Y, Chen Y, Deng H, Wu Q, Xiong J, Meng A, Yu L (2019) Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation. Nat Cell Biol 21(8): 966−977 doi: 10.1038/s41556-019-0358-6

Jiao H, Jiang D, Hu X, Du W, Ji L, Yang Y, Li X, Sho T, Wang X, Li Y, Wu YT, Wei YH, Hu X, Yu L (2021) Mitocytosis, a migrasome-mediated mitochondrial quality-control process. Cell 184(11): 2896−2910 e2813

Liang H, Ma X, Zhang Y, Liu Y, Liu N, Zhang W, Chen J, Liu B, Du W, Liu X, Yu L (2023) The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat Cell Biol 25(8): 1173−1184 doi: 10.1038/s41556-023-01188-8

Liu Y, Li S, Rong W, Zeng C, Zhu X, Chen Q, Li L, Liu ZH, Zen K (2020) Podocyte-released migrasomes in urine serve as an indicator for early podocyte injury. Kidney Dis (Basel) 6(6): 422−433 doi: 10.1159/000511504

Ma L, Li Y, Peng J, Wu D, Zhao X, Cui Y, Chen L, Yan X, Du Y, Yu L (2015) Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res 25(1): 24−38 doi: 10.1038/cr.2014.135

Schmidt-Pogoda A, Strecker JK, Liebmann M, Massoth C, Beuker C, Hansen U, Konig S, Albrecht S, Bock S, Breuer J, Sommer C, Schwab N, Wiendl H, Klotz L, Minnerup J (2018) Dietary salt promotes ischemic brain injury and is associated with parenchymal migrasome formation. PLoS One 13(12): e0209871. https://doi.org/10.1371/journal.pone.0209871

Wang M, Xiong C, Mercurio AM (2022) PD-LI promotes rear retraction during persistent cell migration by altering integrin beta4 dynamics. J Cell Biol 221(5): e202108083. https://doi.org/10.1083/jcb.202108083

Wu D, Xu Y, Ding T, Zu Y, Yang C, Yu L (2017) Pairing of integrins with ECM proteins determines migrasome formation. Cell Res 27(11): 1397−1400 doi: 10.1038/cr.2017.108

Wu J, Lu Z, Jiang D, Guo Y, Qiao H, Zhang Y, Zhu T, Cai Y, Zhang X, Zhanghao K, Xie H, Yan T, Zhang G, Li X, Jiang Z, Lin X, Fang L, Zhou B, Xi P, Fan J, Yu L, Dai Q (2021) Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale. Cell 184(12): 3318−3332 e3317

Wu L, Yang S, Li H, Zhang Y, Feng L, Zhang C, Wei J, Gu X, Xu G, Wang Z, Wang F (2022) TSPAN4-positive migrasome derived from retinal pigmented epithelium cells contributes to the development of proliferative vitreoretinopathy. J Nanobiotechnology 20(1): 519. https://doi.org/10.1186/s12951-022-01732-y

Zhang C, Li T, Yin S, Gao M, He H, Li Y, Jiang D, Shi M, Wang J, Yu L (2022) Monocytes deposit migrasomes to promote embryonic angiogenesis. Nat Cell Biol 24(12): 1726−1738 doi: 10.1038/s41556-022-01026-3

Zhao X, Lei Y, Zheng J, Peng J, Li Y, Yu L, Chen Y (2019) Identification of markers for migrasome detection. Cell Discov 5: 27. https://doi.org/10.1038/s41421-019-0093-y

Zhu M, Zou Q, Huang R, Li Y, Xing X, Fang J, Ma L, Li L, Yang X, Yu L (2021) Lateral transfer of mRNA and protein by migrasomes modifies the recipient cells. Cell Res 31(2): 237−240 doi: 10.1038/s41422-020-00415-3

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