In early pregnancy, approximately 70% of women experience nausea and vomiting, with hyperemesis gravidarum (HG) can potentially lead to severe fluid and nutritional imbalances that require hospitalization. Although HG often resolves on its own in the early stages of pregnancy, its severity is linked to ketosis and elevated serum urea levels, as well as an increased risk of neurodevelopmental disorders in children. To further investigate the immune status of HG patients, we plan to conduct single-cell transcriptomic sequencing and plasma proteomic analysis of peripheral blood samples. This approach aims to elucidate the interactions and mechanisms of PBMCs and provide new insights into potential therapeutic interventions. Our findings indicate an increased proportion of neutrophils in HG patients, along with the upregulation of interferon genes and associated pathways. Notably, the activity of interferon-related TFs, such as STAT1, IRF7, and IRF9, was significantly elevated. Additionally, we observed a decrease in T cell activity in HG patients, while the functionality of NK cells and CD14+ monocytes was enhanced. The elevated plasma levels of NDEL1 may also have implications for fetal development. We have constructed a single-cell atlas of PBMCs from pregnant women with HG, which is expected to enhance our understanding of the immune response in HG and identify potential therapeutic targets for this condition.
","appendixList":[],"articleBusiness":{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","articleState":"-1","articleType":"1","baiduIncludeResult":0,"baiduIncludeResultSearchNum":0,"baiduXueShuIncludeResult":0,"baiduXueShuIncludeResultSearchNum":0,"filename":"2024-064-WJ.xml","googleIncludeResult":0,"googleIncludeResultSearchNum":0,"htmlSource":1,"htmlViewCount":0,"id":"4cfc24b5-2c65-4557-a37b-d4dc66a3ba48","isRegDOI":1,"isUpdate":"1","pdfDownCount":0,"pdfEnFileSizeInt":0,"pdfFileName":"2024-064-WJ.pdf","pdfFileSize":15020.58,"pdfFileSizeInt":15020,"remark":"excel","sortNum":0,"viewCount":0,"xmlFileSize":36.0},"articleNo":"2024-064-WJ","authors":[{"addressTagIds":"aff1","articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","authorNameEn":"Ke Wu","authorRoleType":"author","authorTagVal":"1","authorType":"org","deceased":0,"givenNamesEn":"Ke","id":"6a5279ec-f1cc-4254-be3c-f7aada2338c5","sortNumber":1,"surNameEn":"Wu"},{"addressTagIds":"aff1","articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","authorNameEn":"Xueqiang Yao","authorRoleType":"author","authorTagVal":"1","authorType":"org","deceased":0,"givenNamesEn":"Xueqiang","id":"7efbf364-b074-46ad-bb94-bda1b7c49d0b","sortNumber":2,"surNameEn":"Yao"},{"addressTagIds":"aff1","articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","authorNameEn":"Jie Wen","authorRoleType":"author","authorTagVal":"1","authorType":"org","corresper":true,"correspinfoEn":"jiewen@ustc.edu.cn (J. 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DOI: 10.52601/bpr.2025.240064","doi":"10.52601/bpr.2025.240064","figList":[{"columnNums":2,"dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/5/2024-064-WJ-1.jpg","fileType":"fulltextFig","fileXMLPath":"2024-064-WJ-1.jpg","id":"58f013a9-bbcb-4407-8aac-88063c0f7fbd","imgWidth":"17.0cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"1","nameEn":"Single-cell gene expression profiling of PBMCs in healthy pregnant and HG pregnant women. A Schematic illustration of the study design. B UMAP representation of 41,682 high-quality single cells, where each cluster is assigned a distinct color to denote various sample identities. C UMAP illustration of the distribution across 22 clusters. D Through UMAP analysis of 41,682 high-quality single cells, we identified 8 main clusters of cell types, each differentiated by unique colors. E Violin plots displaying expression levels of marker genes specific to each cell type. F Proportions of each cell type in samples from women with HG and healthy pregnant women. Bars are colored according to cell type","orientation":"idth:17.0cm","referSecTagIds":"","sort":0,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure1","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/5/2024-064-WJ-2.jpg","fileType":"fulltextFig","fileXMLPath":"2024-064-WJ-2.jpg","id":"b9e35663-3af7-4826-ae2e-2996a0ce6df5","imgWidth":"17.0cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"2","nameEn":"Characterization of neutrophils subpopulations in healthy pregnant women and women with HG. A We identified seven main clusters of neutrophils, each distinguished by unique colors. B Violin plots displaying expression levels of marker genes specific to each cell type. C Proportions of each neutrophil in samples from women with HG and healthy pregnant women. Bars are colored according to cell type. D Transcripts from neutrophils were assessed for enrichment of pathway in interferon-stimulated genes (ISG) comparing women with HG and healthy pregnant women. E Comparative assessment of the enrichment of each neutrophil transcript in the ISG pathways. F Comparative analysis of neutrophil transcripts among women with HG, healthy pregnant women, and pregnant women with elevated urinary ketones, assessing the enrichment of transcripts in the ISG pathways. G Heatmap displaying the assessment of different neutrophil types across each group using the Hallmark gene set. H Comparison of neutrophil transcripts between women with HG and healthy pregnant women, assessing the enrichment of transcripts in the neutrophil migration pathways. I Comparative analysis of each neutrophil type across the three groups, assessing the enrichment of neutrophil transcripts in the cell proliferation pathways. Student’s t-test was used to show the statistical difference between the two groups. Significance levels were defined as ns (not significant, P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001","orientation":"idth:17.0cm","referSecTagIds":"","sort":1,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure2","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/5/2024-064-WJ-3.jpg","fileType":"fulltextFig","fileXMLPath":"2024-064-WJ-3.jpg","id":"bde068fd-0bd4-4497-8050-3fbb3db8f913","imgWidth":"16cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"3","nameEn":"Transcription factors and cellular communication related to the interferon pathway in neutrophils of patients with HG. A Rank for regulons in neutrophils based on the RSS. B Heatmap of AUCell values for neutrophil TFs in the three sample groups. Red indicates upregulated TFs, while blue represents downregulated TFs. C Average expression levels of neutrophil TFs across the three sample groups. D Heatmap of AUCell values for neutrophil TFs across each neutrophil subtype in the three sample groups. Red indicates upregulated TFs, while blue represents downregulated TFs. E Bar chart ranking significant signaling pathways based on differences in overall information flow inferred between patients with HG and healthy pregnant women. Red bars represent pathways enriched in healthy pregnant women, while blue bars indicate pathways enriched in patients with HG. F CellChat analysis shows the quantity and strength of interactions among cell types involved in the IFN-II signaling pathway network. G Proportions of receptor-expressing cells mediating potential cellular communication between NK cells and neutrophils via the IFNG-IFNGR1_IFNGR2 pathway in healthy pregnant women and patients with HG","orientation":"idth:16cm","referSecTagIds":"","sort":2,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure3","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/5/2024-064-WJ-4.jpg","fileType":"fulltextFig","fileXMLPath":"2024-064-WJ-4.jpg","id":"20c1af3f-ad43-4bcf-ae06-280d5f222b74","imgWidth":"17.0cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"4","nameEn":"","orientation":"idth:17.0cm","referSecTagIds":"","sort":3,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure4","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/5/2024-064-WJ-5.jpg","fileType":"fulltextFig","fileXMLPath":"2024-064-WJ-5.jpg","id":"14e6324b-6f2c-4258-81aa-227b14f63cae","imgWidth":"17.1cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"5","nameEn":"Subpopulation Characteristics of B Cells, NK Cells, and Monocytes. A Comparison of B cell transcripts between women with HG and healthy pregnant women, assessing the enrichment of transcripts in the B cell receptor signaling pathways. B GO analysis of DEGs in NK cells, highlighting pathway enrichment for upregulated genes. C We identified seven main clusters of NK cells, each distinguished by unique colors. D Comparative assessment of the enrichment of NK cell transcript in the cytotoxic genes. E We identified two main clusters of classical monocytes, each distinguished by unique colors. F Comparative assessment of the enrichment of monocyte transcript in the antigen processing and presentation and complement genes. Student’s t-test was used to show the statistical difference between the two groups. Significance levels were defined as ns (not significant, P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001","orientation":"idth:17.1cm","referSecTagIds":"","sort":4,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure5","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/5/2024-064-WJ-6.jpg","fileType":"fulltextFig","fileXMLPath":"2024-064-WJ-6.jpg","id":"3ef41150-9539-4b84-b569-00669a95ddf4","imgWidth":"17.0cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"6","nameEn":"Proteomics analysis profile. A Distribution of protein quantification abundance dynamic range. The x-axis represents the ranked identified proteins, while the y-axis displays the quantification results of the identified proteins (log10) (left). Box plot of protein quantification results. The x-axis represents sample names, while the y-axis displays the protein quantification results (log10) (right). B Volcano plot of differentially expressed proteins (P-value < 0.05). The x-axis represents the fold change (log2), and the y-axis represents the significance of the differences (−log10 P-value). Red dots indicate significantly upregulated proteins, blue dots represent significantly downregulated proteins, and gray dots correspond to proteins with no significant differences. C Bubble plot of KEGG enrichment analysis for upregulated proteins. The size of the bubbles represents the number of proteins associated with each pathway, and the color indicates the significance level of the enrichment","orientation":"idth:17.0cm","referSecTagIds":"","sort":5,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure6","type":"article","typesetSecTagId":"s03","viewNum":0}],"filePath":"/fileSWWLXB/journal/article/swwlxb/2025/5/","firstFig":{"abstractCn":"","abstractEn":"","contentCn":"","contentEn":"","createTime":"2025-02-19 17:38:40","dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","doi":"","fileLastName":"jpg","fileName":"","filePath":"/fileSWWLXB/journal/article/swwlxb/newcreate/90bbf9ef-7a8f-41ff-bfe4-9ae73b10d737.jpg","fileSize":"126KB","fileType":"firstFig","id":"a04c62db-e92a-4c5a-8911-3f643591cfb0","journalId":"ff007540-a7c7-4752-b593-efa08309babb","link":"","nameCn":"20250219-1","nameEn":"","openTarget":"","remarkCn":"","remarkEn":"","sort":1,"supplementRemarkCn":"","supplementRemarkEn":"","type":"article","viewNum":0},"hasPage":true,"htmlAccess":true,"id":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","issue":"5","issueArticle":"0","keywords":[{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","id":"be1100d8-c6d8-431d-a4f6-f9fe4c47e8a4","keywordEn":"Hyperemesis gravidarum","sortNum":1},{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","id":"836fd385-651f-43b6-a9a7-3308a2ca5b0f","keywordEn":"Pregnancy","sortNum":2},{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","id":"475641e5-e35b-4a48-be79-e19e481d807f","keywordEn":"scRNA-seq","sortNum":3},{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","id":"726e062c-b8a7-4433-9d65-11af34a5291c","keywordEn":"Interferon","sortNum":4},{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","id":"e7ba1a4b-5507-4201-bfbc-f24fbd567888","keywordEn":"PBMCs","sortNum":5}],"language":"en","notes":[],"page":"344-357","pdfAccess":true,"publisherId":"2024-064-WJ","releaseProgress":{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","lastReleaseTime":"2025-11-06 13:54","maxLastReleaseTime":"2025-11-06 13:54","minLastReleaseTime":"2025-11-06 13:54","otherReleaseList":[{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","currentState":"三校","currentStateEn":"In Press","lastReleaseTime":"2025-05-19 14:59","maxLastReleaseTime":"2025-05-19 14:59","minLastReleaseTime":"2025-05-19 14:59","otherReleaseList":[]},{"articleId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","currentState":"最新录用","currentStateEn":"Accepted Manuscript","lastReleaseTime":"2025-02-19 17:20","maxLastReleaseTime":"2025-02-19 17:20","minLastReleaseTime":"2025-02-19 17:20","otherReleaseList":[]}]},"releaseState":1,"searchSort":"20250005000000","subTitleCn":"","subTitleEn":"","supplements":[{"abstractCn":"","abstractEn":"","contentCn":"","contentEn":"","createTime":"2025-02-19 17:38:40","dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","doi":"","fileLastName":"jpg","fileName":"","filePath":"/fileSWWLXB/journal/article/swwlxb/newcreate/90bbf9ef-7a8f-41ff-bfe4-9ae73b10d737.jpg","fileSize":"126KB","fileType":"firstFig","id":"a04c62db-e92a-4c5a-8911-3f643591cfb0","journalId":"ff007540-a7c7-4752-b593-efa08309babb","link":"","nameCn":"20250219-1","nameEn":"","openTarget":"","remarkCn":"","remarkEn":"","sort":1,"supplementRemarkCn":"","supplementRemarkEn":"","type":"article","viewNum":0}],"tableList":[],"tags":[{"data":"{\"publisherId\":\"\",\"journalName\":\"\",\"remark\":\"\",\"createDate\":\"\",\"createUser\":\"\",\"status\":\"1\"}","id":"0","journalId":"ff007540-a7c7-4752-b593-efa08309babb","level":1,"nameCn":"轮播推荐","nameEn":"轮播推荐","outputName":"banner_recommend","remark":"","sort":0,"tags":[],"type":"recommend"},{"id":"b659be25-0494-476c-9da2-bc5144781a33","journalId":"ff007540-a7c7-4752-b593-efa08309babb","level":1,"nameCn":"Controlled Nanomedicine Materials","nameEn":"Controlled Nanomedicine Materials","outputName":"d7f3587a-4fc9-43ed-a962-5419863a39f0","sort":0,"tags":[],"type":"topic"}],"titleCn":"","titleEn":"Single-cell atlas of peripheral blood mononuclear cells in pregnant women with hyperemesis gravidarum","topicNameEn":"","type":"research-article","volume":"11","year":"2025","yearInt":2025},"dataId":"0162adb4-7d1f-4c87-8a41-bfee9c03c73b","dataType":"Article","id":"4b0442b0-8eca-4c27-9f45-eee84a6a4ce6","language":"cn,en","sort":12,"tagId":"0"},{"data":{"abstractAccess":true,"abstractinfoCn":"","abstractinfoEn":"Abnormal amyloid fibrils are characteristic features and common pathological mechanisms of various neurodegenerative diseases, often found in disease-related brain regions, leading to neuroinflammation and neuronal apoptosis. Many disease-associated amyloid fibrils consist of a rigid fibril core primarily composed of cross-β sheets, surrounded by a fuzzy coat formed by intrinsically disordered regions (IDR). Over the past two decades, substantial structural knowledge of the rigid fibril core has been accumulated through cryo-electron microscopy (cryo-EM) and solid-state nuclear magnetic resonance (ssNMR) based on cross-polarization. In contrast, the highly disordered conformations of the fuzzy coats have hindered their structural characterization. Here, we describe the application of two-dimensional (2D) heteronuclear single quantum coherence (HSQC) and three-dimensional (3D) HNCO, HNCA, and HN(CO)CA spectra, utilizing the scalar coupling-based 1H detection magic angle spinning (MAS) ssNMR techniques for backbone assignment of the IDR in amyloid fibrils, with the aim of further elucidating the conformational changes of the IDR during ligand binding processes.
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Apart from the 13C carrier frequency, both pulse sequences are identical. The 90° pulse in the carbon channel is a Q5-shaped pulse of 400 µs duration, while the 180° pulse is a Q3-shaped pulse of 256 µs duration. C Schematic diagram of the 3D HN(CO)CA experiment pulse sequence, with τ, T and δ set to 2.3 ms,12.0 ms and 4 ms, respectively. The 90° pulse in the carbon channel is a Q5-shaped pulse of 400 µs duration, while the 180° pulse is a Q3-shaped pulse of 256 µs duration","orientation":"idth:17.0cm","referSecTagIds":"","sort":1,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure2","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"71b411a2-6bdc-4f9b-bff1-50e51df5d549","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/4/2024-065-XSQ-3.jpg","fileType":"fulltextFig","fileXMLPath":"2024-065-XSQ-3.jpg","id":"5ab7692b-3ffd-469e-bb77-d1e65cfeb0bd","imgWidth":"17.0cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"3","nameEn":"Schematic diagram of the spin polarization transfer pathways in the 3D HNCO, HNCA, and HN(CO)CA experiments, highlighting two adjacent residues (residues i-1 and i) within the protein backbone. Arrows indicate the polarization transfer process. Colored circles represent the nuclei for which chemical shifts were correlated during the experiment","orientation":"idth:17.0cm","referSecTagIds":"","sort":2,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure3","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":1,"dataId":"71b411a2-6bdc-4f9b-bff1-50e51df5d549","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/4/2024-065-XSQ-4.jpg","fileType":"fulltextFig","fileXMLPath":"2024-065-XSQ-4.jpg","id":"b1af198a-c7e8-42b0-a763-957d5898ffe2","imgWidth":"8.0cm","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"4","nameEn":"Overlay of the 2D 1H-15N projection of the 3D HN(CO)CA spectrum (turquoise) onto the 2D 1H-15N refocused-HSQC spectrum (black). The non-overlapping peaks at the lower right may be due to sample degradation during the experimental process","orientation":"idth:8.0cm","referSecTagIds":"","sort":3,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure4","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"71b411a2-6bdc-4f9b-bff1-50e51df5d549","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/4/2024-065-XSQ-5.jpg","fileType":"fulltextFig","fileXMLPath":"2024-065-XSQ-5.jpg","id":"882cef31-febe-4e44-a6f4-c4afad77df69","imgWidth":"12","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"5","nameEn":"A 2D 1H-13CO projection of the 3D HNCO spectrum of uniformly 15N/13C-labeled α-Syn fibrils recorded in a 1.3-mm rotor at a 600-MHz spectrometer at 60-kHz MAS, illustrating the signal pattern of the sample. B 2D 1H-13CA projection of the 3D HNCA spectrum. C 2D 1H-13CA projection of the 3D HN(CO)CA spectrum. It is noteworthy that the signals appearing in the 1H dimension at 7.8–7.6 ppm are attributed to sample degradation","orientation":"idth:12","referSecTagIds":"","sort":4,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure5","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"71b411a2-6bdc-4f9b-bff1-50e51df5d549","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/4/2024-065-XSQ-6.jpg","fileType":"fulltextFig","fileXMLPath":"2024-065-XSQ-6.jpg","id":"2cbe13f1-3db1-4be0-bd72-447bd06db7f7","imgWidth":"13","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"6","nameEn":"A 2D 1H-15N refocused-HSQC spectrum of uniformly 15N/13C-labeled α-Syn fibrils recorded in a 1.3-mm rotor at a 600-MHz spectrometer at 60-kHz MAS, with assigned residues marked. The clustering of residue signals in the 7.8 to 8.6 ppm range indicates the detection of the IDR of α-Syn fibrils. B 2D 1H-15N HSQC spectrum of uniformly 15N/13C-labeled α-Syn monomers. This is adapted with permission from Zhang et al. (2023)","orientation":"idth:13","referSecTagIds":"","sort":5,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure6","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":2,"dataId":"71b411a2-6bdc-4f9b-bff1-50e51df5d549","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/4/2024-065-XSQ-7.jpg","fileType":"fulltextFig","fileXMLPath":"2024-065-XSQ-7.jpg","id":"5ef6eb6d-74b9-461f-b379-adec466c9d0a","imgWidth":"15","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"7","nameEn":"Sequential backbone walks from N122 to M127 of the 15N/13C-labeled α-Syn fibrils, achieved through paired HN(CO)CA (left, blue) and HNCA (right, red) experiments. Each strip is 0.2 ppm wide. The HNCA and HN(CO)CA spectra can complete the assignment of most signals in the HSQC spectrum of α-Syn fibrils. This is adapted with permission from Zhang et al. (2023)","orientation":"idth:15","referSecTagIds":"","sort":6,"supplementRemarkCn":"","supplementRemarkEn":"","tagId":"Figure7","type":"article","typesetSecTagId":"s03","viewNum":0},{"columnNums":1,"dataId":"71b411a2-6bdc-4f9b-bff1-50e51df5d549","fileFrom":"xml","fileLastName":"jpg","filePath":"/fileSWWLXB/journal/article/swwlxb/2025/4/2024-065-XSQ-8.jpg","fileType":"fulltextFig","fileXMLPath":"2024-065-XSQ-8.jpg","id":"c0fa47e2-7224-4022-b8f8-60babec08b6c","imgWidth":"7","journalId":"ff007540-a7c7-4752-b593-efa08309babb","labelText":"8","nameEn":"Overlay of the 2D 1H-15N refocused-HSQC spectra of 15N/13C-labeled α-Syn fibrils in the absence (black) and presence of L3D1 (green), with an α-Syn to L3D1 molar ratio of 1:0.5. Arrows indicate several prominent spectral peaks that have disappeared. This is adapted with permission from Zhang et al. 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